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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of a novel lipase from the digestive glands of a primitive animal: The scorpion (2005), Biochim. Biophys. Acta, 1726, 67-74.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Scorpio maurus | |
| 17 | - |
tributyrin | pH 9.0, 37°C | Scorpio maurus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme contains a single SH group accessible to sulfhydryl reagents and not essential for activity | Scorpio maurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Scorpio maurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scorpio maurus | - |
collected in Tunisia | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sugar content about 15% of the molecular mass | Scorpio maurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 506fold from scorpion digestive glands by anion exchange chromatography, ammonium sulfate fractionation, gel filtration, and cation exchange chromatography to homogeneity | Scorpio maurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | the enzyme contains an active site serine residue | Scorpio maurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | - |
Scorpio maurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
titrimetric assay method | Scorpio maurus |
| 5023 | - |
purified enzyme | Scorpio maurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tributyrin + H2O | - |
Scorpio maurus | dibutyrin + butyrate | - |
? | |
| triolein + H2O | olive oil | Scorpio maurus | diolein + oleate | - |
? | |
| tripropionin + H2O | - |
Scorpio maurus | dipropionin + propionate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, SDS-PAGE | Scorpio maurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Scorpio maurus |
| scorpio digestive lipase | - |
Scorpio maurus |
| SDL | - |
Scorpio maurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Scorpio maurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
inactivation above within 5 min | Scorpio maurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5370 | - |
tributyrin | pH 9.0, 37°C | Scorpio maurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Scorpio maurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | inactive below pH 5.0 | Scorpio maurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
4 h, 65% remaining activity | Scorpio maurus |
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