KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Scorpio maurus | |
17 | - |
tributyrin | pH 9.0, 37°C | Scorpio maurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains a single SH group accessible to sulfhydryl reagents and not essential for activity | Scorpio maurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Scorpio maurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scorpio maurus | - |
collected in Tunisia | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | sugar content about 15% of the molecular mass | Scorpio maurus |
Purification (Comment) | Organism |
---|---|
native enzyme 506fold from scorpion digestive glands by anion exchange chromatography, ammonium sulfate fractionation, gel filtration, and cation exchange chromatography to homogeneity | Scorpio maurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | the enzyme contains an active site serine residue | Scorpio maurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive gland | - |
Scorpio maurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
titrimetric assay method | Scorpio maurus |
5023 | - |
purified enzyme | Scorpio maurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tributyrin + H2O | - |
Scorpio maurus | dibutyrin + butyrate | - |
? | |
triolein + H2O | olive oil | Scorpio maurus | diolein + oleate | - |
? | |
tripropionin + H2O | - |
Scorpio maurus | dipropionin + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Scorpio maurus |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Scorpio maurus |
scorpio digestive lipase | - |
Scorpio maurus |
SDL | - |
Scorpio maurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Scorpio maurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
inactivation above within 5 min | Scorpio maurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5370 | - |
tributyrin | pH 9.0, 37°C | Scorpio maurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Scorpio maurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | inactive below pH 5.0 | Scorpio maurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
11 | - |
4 h, 65% remaining activity | Scorpio maurus |