Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dimethyl formamide | concentrated solution increases the enzyme activity 2fold, maximal activity at a water content of 0.5-1% w/w | Pseudomonas aeruginosa | |
dimethyl sulfoxide | concentrated solution increases the enzyme activity 5fold, maximal activity at a water content of 0.5-1% w/w | Pseudomonas aeruginosa | |
additional information | alcohols and glycols up to 20% activate the enzyme, high concentrations inhibit, the extracellular enzyme is induced by triolein, soybean oil, Tween 20, Tween 80, and especially by free fatty acids, e.g. oleic acid or lauric acid more than 25fold | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | - |
Pseudomonas aeruginosa | |
additional information | alcohols and glycols up to 20% activates the enzyme, high concentrations inhibit | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion of the enzyme to culture medium | Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, about, SDS-PAGE | Pseudomonas aeruginosa |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl formamide | the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide | Pseudomonas aeruginosa |
dimethyl sulfoxide | the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide | Pseudomonas aeruginosa |
additional information | the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethylsulfoxide | Pseudomonas aeruginosa |
Pyridine | the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethylsulfoxide | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa YS-7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from culture supernatant by acetone precipitation and hydrophobic interaction chromatography | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | culture conditions evaluation, overview | Pseudomonas aeruginosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.22 | - |
purified enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | high activity in a water-restricted environment containing a water content of 0.5-1% w/w | Pseudomonas aeruginosa | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | high activity in a water-restricted environment containing a water content of 0.5-1% w/w | Pseudomonas aeruginosa YS-7 | 4-nitrophenol + laurate | - |
? | |
soybean oil + H2O | concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w | Pseudomonas aeruginosa | ? | - |
? | |
soybean oil + H2O | concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w | Pseudomonas aeruginosa YS-7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, about, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | purified enzyme, stable for over 30 days in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas aeruginosa |