Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hexadecyl trimethyl ammonium bromide | strong activation | Aspergillus carneus | |
additional information | no effect by metal chelators | Aspergillus carneus | |
n-octyl-alpha-D-glucopyranoside | strong activation | Aspergillus carneus | |
n-octyl-beta-D-glucopyranoside | strong activation | Aspergillus carneus | |
taurocholic acid | strong activation, 2.7fold at 10 mM | Aspergillus carneus |
General Stability | Organism |
---|---|
half-life of the enzyme is enhanced by glycine, sorbitol, glycerol, glucose, and ammonium chloride | Aspergillus carneus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bromosuccinimide | - |
Aspergillus carneus | |
cholic acid | - |
Aspergillus carneus | |
diethyl 4-nitrophenyl phosphate | strong inhibition | Aspergillus carneus | |
additional information | no effect by metal chelators, no inhibition by trinitrobenzene sulfonic acid, N-ethylmaleimide, p-chloromercuribenzoate, o-phenanthroline, and 4,4'-dipyridyl-disulfide | Aspergillus carneus | |
N-lauroyl sarcosine | strong inhibition | Aspergillus carneus | |
PMSF | strong inhibition | Aspergillus carneus | |
SDS | strong inhibition | Aspergillus carneus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme follows first order reaction kinetics during inactivation | Aspergillus carneus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | acivation | Aspergillus carneus | |
Na+ | acivation | Aspergillus carneus | |
NH4+ | acivation | Aspergillus carneus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
about, native PAGE | Aspergillus carneus |
Organic Solvent | Comment | Organism |
---|---|---|
Butanol | 80% loss of activity after 30 min, complete loss of activity after 24 h | Aspergillus carneus |
Ethanol | complete loss of activity after 30 min in 90% ethanol, loss of 41% activity after 30 min in 40% ethanol | Aspergillus carneus |
additional information | the enzyme is highly active in presence of many different organic solvents, overview | Aspergillus carneus |
propan-2-ol | 97% loss of activity after 30 min in 60% propan-2-ol, loss of only 3% activity after 30 min in 30% propan-2-ol | Aspergillus carneus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus carneus | - |
alkaline thermostable lipase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 12.3% glycosylated | Aspergillus carneus |
Purification (Comment) | Organism |
---|---|
24.1fold | Aspergillus carneus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | a tryptophan residue plays an important role in maintaining the ative conformation of the enzyme | Aspergillus carneus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
502 | - |
purified enzyme | Aspergillus carneus |
Storage Stability | Organism |
---|---|
22°C, room temperature, lyophilized enzyme, completely stable for more than 1 year | Aspergillus carneus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | low activity | Aspergillus carneus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Aspergillus carneus | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl laurate + H2O | enzyme shows a preference for lauric acid and a 1,3-position specificity | Aspergillus carneus | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | - |
Aspergillus carneus | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Aspergillus carneus | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | low activity | Aspergillus carneus | 4-nitrophenol + stearate | - |
? | |
additional information | the enzyme utilizes olive oil as a substrate, no activity with 2-nitrophenyl palmitate | Aspergillus carneus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, SDS-PAGE | Aspergillus carneus |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Aspergillus carneus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Aspergillus carneus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 90 | - |
Aspergillus carneus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability is enhanced by glycine, sorbitol, glycerol, glucose, and ammonium chloride | Aspergillus carneus |
70 | - |
5 min, stable, after 10 min enzyme shows loss of 45% activity | Aspergillus carneus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Aspergillus carneus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus carneus | isoelectric focusing | 4.4 | 4.3 |