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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of an extracellular lipase from Penicillium candidum (2001), Lipids, 36, 283-289.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation | Penicillium candidum |  |
| Nonidet P-40 | - |
Penicillium candidum | |
| nonionic detergents | stimulation | Penicillium candidum | |
| Triton X-100 | - |
Penicillium candidum | |
| Tween 20 | - |
Penicillium candidum | |
| Zwittergent | - |
Penicillium candidum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | about 75% inhibition at 1 mM | Penicillium candidum | |
| Fe2+ | complete inhibition at 1 mM | Penicillium candidum | |
| ionic detergents | - |
Penicillium candidum | |
| sodium cholate | - |
Penicillium candidum | |
| Tween 80 | - |
Penicillium candidum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Penicillium candidum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
1 * 29000, SDS-PAGE | Penicillium candidum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium candidum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 36.7fold, to homogeneity | Penicillium candidum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13900 | - |
purified enzyme, substrate 4-nitrophenyl laurate | Penicillium candidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | low activity | Penicillium candidum | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl decanoate + H2O | - |
Penicillium candidum | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | low activity | Penicillium candidum | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Penicillium candidum | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl octanoate + H2O | low activity | Penicillium candidum | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl palmitate + H2O | best substrate | Penicillium candidum | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl stearate + H2O | - |
Penicillium candidum | 4-nitrophenol + stearate | - |
? | |
| additional information | substrate specificity | Penicillium candidum | ? | - |
? | |
| tributyrin + H2O | best substrate | Penicillium candidum | dibutyrin + butyrate | - |
? | |
| tridecanin + H2O | - |
Penicillium candidum | didecanin + decanoate | - |
? | |
| tridodecanin + H2O | - |
Penicillium candidum | didodecanin + dodecanoate | - |
? | |
| trihexanin + H2O | - |
Penicillium candidum | dihexanin + hexanoate | - |
? | |
| trimyristin + H2O | - |
Penicillium candidum | dimyristin + myristate | - |
? | |
| triolein + H2O | - |
Penicillium candidum | diolein + oleate | - |
? | |
| tripalmitin + H2O | - |
Penicillium candidum | dipalmitin + palmitate | - |
? | |
| tristearin + H2O | - |
Penicillium candidum | distearin + stearate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE | Penicillium candidum |
| More | the enzyme forms dimers and higher molecular weight aggregates under denaturing conditions | Penicillium candidum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Penicillium candidum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Penicillium candidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
above, rapid loss of activity | Penicillium candidum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Penicillium candidum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penicillium candidum | isoelectric focusing | - |
5.5 |
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