Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sorbitol | 50%, 30 min, 70°C, pH 8.5, 10% activation | Geobacillus stearothermophilus |
Application | Comment | Organism |
---|---|---|
synthesis | possible industrial application of the enzyme in continuous processes due to effective immobilization on diverse materials, overview | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 30 min, 70°C, pH 8.5, 65% inhibition | Geobacillus stearothermophilus | |
Cu2+ | 5 mM, 30 min, 70°C, pH 8.5, complete inhibition | Geobacillus stearothermophilus | |
dithiothreitol | 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition | Geobacillus stearothermophilus | |
Fe2+ | 5 mM, 30 min, 70°C, pH 8.5, 90% inhibition | Geobacillus stearothermophilus | |
Hg2+ | 5 mM, 30 min, 70°C, pH 8.5, 55% inhibition | Geobacillus stearothermophilus | |
Li+ | 5 mM, 30 min, 70°C, pH 8.5, 15% inhibition | Geobacillus stearothermophilus | |
Mg2+ | 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition | Geobacillus stearothermophilus | |
Mn2+ | 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition | Geobacillus stearothermophilus | |
additional information | no inhibition by iodoacetamide, K+, Na+ at 5 mM | Geobacillus stearothermophilus | |
N-bromosuccinimide | 5 mM, 30 min, 70°C, pH 8.5, 55% inhibition | Geobacillus stearothermophilus | |
p-chloromercuribenzoate | 5 mM, 30 min, 70°C, pH 8.5, 70% inhibition | Geobacillus stearothermophilus | |
PMSF | 5 mM, 30 min, 70°C, pH 8.5, complete inhibition | Geobacillus stearothermophilus | |
Zn2+ | 5 mM, 30 min, 70°C, pH 8.5, 85% inhibition | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
4-nitrophenyl palmitate | pH 8.5, 60°C | Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacillus stearothermophilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10% stimulation at 5 mM | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62500 | - |
x * 62500, SDS-PAGE | Geobacillus stearothermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 50%, 30 min, 70°C, pH 8.5, loss of 60% activity | Geobacillus stearothermophilus |
Butanol | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
chloroform | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
diethyl ether | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
Ethanol | 50%, 30 min, 70°C, pH 8.5, loss of 70% activity | Geobacillus stearothermophilus |
Glycerol | 50%, 30 min, 70°C, pH 8.5, loss of 60% activity | Geobacillus stearothermophilus |
isopropanol | 50%, 30 min, 70°C, pH 8.5, loss of 60% activity | Geobacillus stearothermophilus |
Methanol | 50%, 30 min, 70°C, pH 8.5, loss of 20% activity | Geobacillus stearothermophilus |
n-hexane | 50%, 30 min, 70°C, pH 8.5, loss of 85% activity | Geobacillus stearothermophilus |
sorbitol | 50%, 30 min, 70°C, pH 8.5, 10% activation | Geobacillus stearothermophilus |
toluol | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
thermostable lipase | - |
Geobacillus stearothermophilus MC 7 | - |
thermostable lipase | - |
Purification (Comment) | Organism |
---|---|
19.25fold, from culture supernatant | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.9 | - |
purified enzyme, substrate 4-nitrophenyl palmitate | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus MC 7 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl palmitate + H2O | best substrate | Geobacillus stearothermophilus | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | best substrate | Geobacillus stearothermophilus MC 7 | 4-nitrophenol + palmitate | - |
? | |
DL-alpha-palmitin + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
DL-alpha-stearin + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
additional information | the enzyme also possesses esterase activity | Geobacillus stearothermophilus | ? | - |
? | |
additional information | the enzyme also possesses esterase activity | Geobacillus stearothermophilus MC 7 | ? | - |
? | |
olive oil + H2O | emulsion | Geobacillus stearothermophilus | ? | - |
? | |
triacetin + H2O | - |
Geobacillus stearothermophilus | diacetin + acetate | - |
? | |
tributyrin + H2O | best substrate | Geobacillus stearothermophilus | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | best substrate | Geobacillus stearothermophilus MC 7 | dibutyrin + butyrate | - |
? | |
tricaprin + H2O | - |
Geobacillus stearothermophilus | dicaprin + capriate | - |
? | |
trilaurin + H2O | - |
Geobacillus stearothermophilus | dilaurin + laurate | - |
? | |
triolein + H2O | - |
Geobacillus stearothermophilus | diolein + oleate | - |
? | |
triolein + H2O | - |
Geobacillus stearothermophilus MC 7 | diolein + oleate | - |
? | |
tripalmitin + H2O | - |
Geobacillus stearothermophilus | dipalmitin + palmitate | - |
? | |
tristearin + H2O | - |
Geobacillus stearothermophilus | distearin + stearate | - |
? | |
Tween 80 + H2O | i.e. poly(oxyethylene) sorbitan monooleate | Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62500, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | - |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 90 | - |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified enzyme: half-life is 30 min, crude enzyme: half-life is 3 h | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Geobacillus stearothermophilus |