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Literature summary for 3.1.1.3 extracted from
- Isolation, purification, and characterization of a cold-active lipase from Aspergillus nidulans (2000), J. Agric. Food Chem., 48, 105-109.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
4-nitrophenyl palmitate | pH 6.5, 40°C | Aspergillus nidulans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | culture medium | Aspergillus nidulans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Aspergillus nidulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | Aspergillus nidulans | - |
4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | Aspergillus nidulans WG312 | - |
4-nitrophenol + palmitate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
cultured with olive oil as carbon source or in rich medium | - |
| Aspergillus nidulans WG312 | - |
cultured with olive oil as carbon source or in rich medium | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Aspergillus nidulans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from culture medium, to homogeneity, 1186fold | Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity at different culture medium conditions | Aspergillus nidulans |
| 355.7 | - |
purified enzyme | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Aspergillus nidulans | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Aspergillus nidulans WG312 | 4-nitrophenol + palmitate | - |
? | |
| additional information | substrate specificity | Aspergillus nidulans | ? | - |
? | |
| additional information | substrate specificity | Aspergillus nidulans WG312 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus nidulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
20 | cold-adapted enzyme, high activity in the range 0-20°C | Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
cold-adapted enzyme shows reduced thermal stability | Aspergillus nidulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 494 | - |
4-nitrophenyl palmitate | pH 6.5, 40°C | Aspergillus nidulans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Aspergillus nidulans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus nidulans | isoelectric focusing | - |
4.85 |
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