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Literature summary for 3.1.1.3 extracted from

  • Fernandez, J.; Mohedano, A.F.; Fernandez-Garcia, E.; Medina, M.; Nunez, M.
    Purification and characterization of an extracellular tributyrin esterase produced by a cheese isolate, Micrococcus sp. INIA 528 (2004), Int. Dairy J., 14, 135-142.
    View publication on PubMed
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol strong inhibition Micrococcus sp.
Co2+
-
 Micrococcus sp.
Cu2+
-
 Micrococcus sp.
cysteine
-
 Micrococcus sp.
dithiothreitol strong inhibition Micrococcus sp.
EDTA strong inhibition Micrococcus sp.
Fe2+
-
 Micrococcus sp.
Mn2+ slight inhibition Micrococcus sp.
additional information no inhibition by PMSF, E-64, 1,10-phenanthroline, and tosyllysylchloromethyl ketone Micrococcus sp.
NaCl loss of 50% activity at 7.5% Micrococcus sp.
Ni2+
-
 Micrococcus sp.
Zn2+
-
 Micrococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 alpha-naphthyl caprylate pH 7.5, 30°C Micrococcus sp.
0.08
-
 alpha-naphthyl butyrate pH 7.5, 30°C Micrococcus sp.
0.6
-
 tricaprylin pH 7.5, 30°C Micrococcus sp.
5
-
 tributyrin pH 7.5, 30°C Micrococcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion to the culture medium Micrococcus sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Mg2+, Ca2+, K+, Na+ Micrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44600
-
 x * 44600, SDS-PAGE Micrococcus sp.

Organism

Organism UniProt Comment Textmining
Micrococcus sp.
-
-
-
Micrococcus sp. INIA 528
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant to homogeneity, 296fold Micrococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
239.5
-
 purified enzyme Micrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-naphthyl butyrate + H2O preferred substrate Micrococcus sp. alpha-naphthol + butyrate
-
 ?
alpha-naphthyl butyrate + H2O preferred substrate Micrococcus sp. INIA 528 alpha-naphthol + butyrate
-
 ?
alpha-naphthyl caprylate + H2O 58% of the activity with the alpha-isomer Micrococcus sp. alpha-naphthol + caprylate
-
 ?
alpha-naphthyl caprylate + H2O 58% of the activity with the alpha-isomer Micrococcus sp. INIA 528 alpha-naphthol + caprylate
-
 ?
alpha-naphthyl laurate + H2O
-
 Micrococcus sp. alpha-naphthol + laurate
-
 ?
alpha-naphthyl stearate + H2O equally active with both isomers Micrococcus sp. alpha-naphthol + stearate
-
 ?
beta-naphthyl butyrate + H2O 58% of the activity with the alpha-isomer Micrococcus sp. beta-naphthol + butyrate
-
 ?
beta-naphthyl caprylate + H2O
-
 Micrococcus sp. beta-naphthol + caprylate
-
 ?
beta-naphthyl laurate + H2O 62.5% of the activity with the alpha-isomer Micrococcus sp. beta-naphthol + laurate
-
 ?
beta-naphthyl stearate + H2O equally active with both isomers Micrococcus sp. beta-naphthol + stearate
-
 ?
additional information the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 Micrococcus sp. ?
-
 ?
additional information the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 Micrococcus sp. INIA 528 ?
-
 ?
tributyrin + H2O best substrate Micrococcus sp. dibutyrin + butyrate
-
 ?
tributyrin + H2O best substrate Micrococcus sp. INIA 528 dibutyrin + butyrate
-
 ?
tricaprylin + H2O
-
 Micrococcus sp. dicaprylin + caprylate
-
 ?
trilaurin + H2O
-
 Micrococcus sp. dilaurin + laurate
-
 ?
trilaurin + H2O
-
 Micrococcus sp. INIA 528 dilaurin + laurate
-
 ?

Subunits

Subunits Comment Organism
? x * 44600, SDS-PAGE Micrococcus sp.

Synonyms

Synonyms Comment Organism
tributyrin esterase
-
 Micrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Micrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 80% remaining activity Micrococcus sp.
50
-
 5 min, 50% remaining activity Micrococcus sp.
60 70 less than 20% activity after 2 min Micrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Micrococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 rapid decrease in activity below pH 6.0 and above pH 8.5 Micrococcus sp.