Cloned (Comment) | Organism |
---|---|
gene lipA, expression in Bacillus megaterium WH320 | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion to the medium, native and recombinant enzyme | Bacillus subtilis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes ssp. | - |
commercial product | - |
Bacillus subtilis | - |
gene lipA | - |
Bacillus subtilis 168 BsL | - |
gene lipA | - |
Chromobacterium viscosum | - |
commercial product | - |
Diutina rugosa | - |
type VII, commercial product | - |
Fusarium solani | - |
cutinase, commercial product, 90% pure enzyme | - |
Homo sapiens | - |
- |
- |
Rhizomucor miehei | - |
commercial product | - |
Rhizopus arrhizus | - |
commercial product | - |
Rhizopus japonicus | - |
commercial product | - |
Sus scrofa | - |
3 commercially available enzymes, PPL | - |
Thermomyces lanuginosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of the commercial product from elastin | Sus scrofa |
recombinant extracellular enzyme, expressed in Bacillus megaterium, from culture supernatant, to more than 90% purity | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Homo sapiens | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Sus scrofa | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Rhizopus arrhizus | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Diutina rugosa | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Thermomyces lanuginosus | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Rhizopus japonicus | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Chromobacterium viscosum | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases | Alcaligenes ssp. | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases, enzyme contains the alpha-helical lid structure | Rhizomucor miehei | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases, lipase contains no lid structure | Fusarium solani | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad consists of Ser, His, and Asp residues, as for serine proteases, the enzyme contains no lid structure | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cartilage | elastin, commercial product | Sus scrofa | - |
pancreas | - |
Homo sapiens | - |
pancreas | 2 commercially available enzymes | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenol butyrate + H2O | - |
Bacillus subtilis | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenol butyrate + H2O | - |
Bacillus subtilis 168 BsL | 4-nitrophenol + butyrate | - |
? | |
additional information | enzyme also utilizes water-soluble substrates, enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Fusarium solani | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Bacillus subtilis | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Homo sapiens | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Sus scrofa | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Rhizopus arrhizus | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Diutina rugosa | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Rhizomucor miehei | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Thermomyces lanuginosus | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Rhizopus japonicus | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Chromobacterium viscosum | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Alcaligenes ssp. | ? | - |
? | |
additional information | enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide | Bacillus subtilis 168 BsL | ? | - |
? | |
tributyrin + H2O | - |
Bacillus subtilis | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Homo sapiens | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Sus scrofa | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Rhizopus arrhizus | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Fusarium solani | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Diutina rugosa | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Rhizomucor miehei | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Thermomyces lanuginosus | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Rhizopus japonicus | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Chromobacterium viscosum | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Alcaligenes ssp. | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Bacillus subtilis 168 BsL | dibutyrin + butyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cutinase | - |
Fusarium solani |
lipase | - |
Bacillus subtilis |
lipase | - |
Homo sapiens |
lipase | - |
Sus scrofa |
lipase | - |
Rhizopus arrhizus |
lipase | - |
Diutina rugosa |
lipase | - |
Rhizomucor miehei |
lipase | - |
Thermomyces lanuginosus |
lipase | - |
Rhizopus japonicus |
lipase | - |
Chromobacterium viscosum |
lipase | - |
Alcaligenes ssp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus subtilis |
25 | - |
assay at | Homo sapiens |
25 | - |
assay at | Sus scrofa |
25 | - |
assay at | Rhizopus arrhizus |
25 | - |
assay at | Fusarium solani |
25 | - |
assay at | Diutina rugosa |
25 | - |
assay at | Rhizomucor miehei |
25 | - |
assay at | Thermomyces lanuginosus |
25 | - |
assay at | Rhizopus japonicus |
25 | - |
assay at | Chromobacterium viscosum |
25 | - |
assay at | Alcaligenes ssp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis |
7 | - |
assay at | Homo sapiens |
7 | - |
assay at | Sus scrofa |
7 | - |
assay at | Rhizopus arrhizus |
7 | - |
assay at | Fusarium solani |
7 | - |
assay at | Diutina rugosa |
7 | - |
assay at | Rhizomucor miehei |
7 | - |
assay at | Thermomyces lanuginosus |
7 | - |
assay at | Rhizopus japonicus |
7 | - |
assay at | Chromobacterium viscosum |
7 | - |
assay at | Alcaligenes ssp. |