General Stability | Organism |
---|---|
Ca2+ stabilizes the enzyme | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction kinetics in a monomolecular layer of different diacylglycerides | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted to the culture medium | Aspergillus niger | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger Tiegh | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from culture supernatant, to homogeneity | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
729 | - |
purified enzyme, substrate tricaprylin | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with trielaidin, an olive oil emulsion containing 10% v/v olive oil and 1% m/v gum arabic is used as substrate, the enzyme shows a sn-1 selectivity using diacylglycerols, and R-isomer hydrolytic preference with pseudolipids representing triacylglycerols in which 2 of the ester bonds are replaced with ether and amide linkages | Aspergillus niger | ? | - |
? | |
additional information | no activity with trielaidin, an olive oil emulsion containing 10% v/v olive oil and 1% m/v gum arabic is used as substrate, the enzyme shows a sn-1 selectivity using diacylglycerols, and R-isomer hydrolytic preference with pseudolipids representing triacylglycerols in which 2 of the ester bonds are replaced with ether and amide linkages | Aspergillus niger Tiegh | ? | - |
? | |
triacetin + H2O | - |
Aspergillus niger | diacetin + acetate | - |
? | |
tributyrin + H2O | - |
Aspergillus niger | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Aspergillus niger Tiegh | dibutyrin + butyrate | - |
? | |
tricaproin + H2O | - |
Aspergillus niger | dicaproin + caproate | - |
? | |
tricaprylin + H2O | best substrate | Aspergillus niger | dicaprylin + caprylate | - |
? | |
tricaprylin + H2O | best substrate | Aspergillus niger Tiegh | dicaprylin + caprylate | - |
? | |
trilinolein + H2O | trans C18:1(9) | Aspergillus niger | dilinolein + linoleate | - |
? | |
trilinolein + H2O | trans C18:1(9) | Aspergillus niger Tiegh | dilinolein + linoleate | - |
? | |
triolein + H2O | cis-C18:1(9) | Aspergillus niger | diolein + oleate | - |
? | |
triolein + H2O | cis-C18:1(9) | Aspergillus niger Tiegh | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | - |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | - |
Aspergillus niger |