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Literature summary for 3.1.1.3 extracted from

  • Arreguin-Espinosa, R.; Arreguin, B.; Gonzalez, C.
    Purification and properties of a lipase from Cephaloleia presignis (Coleoptera, Chrysomelidae) (2000), Biotechnol. Appl. Biochem., 31, 239-244.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information not affected by Ca2+, Mn2+, Mg2+, Na+, K+, PMSF, EDTA, all at 1 mM Cephaloleia presignis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition, 1 mM Cephaloleia presignis
Cu2+ complete inhibition, 1 mM Cephaloleia presignis
diisopropyl fluorophosphate complete inhibition, 1 mM Cephaloleia presignis
Fe2+ complete inhibition, 1 mM Cephaloleia presignis
Fe3+ complete inhibition, 1 mM Cephaloleia presignis
additional information not affected by Ca2+, Mn2+, Mg2+, Na+, K+, PMSF, EDTA, all at 1 mM Cephaloleia presignis
Zn2+ complete inhibition, 1 mM Cephaloleia presignis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular hemolymph Cephaloleia presignis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metalloenzyme Cephaloleia presignis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
gel filtration Cephaloleia presignis
31000
-
1 * 31000, SDS-PAGE Cephaloleia presignis

Organism

Organism UniProt Comment Textmining
Cephaloleia presignis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Cephaloleia presignis

Purification (Commentary)

Purification (Comment) Organism
20.3fold Cephaloleia presignis

Source Tissue

Source Tissue Comment Organism Textmining
hemolymph
-
Cephaloleia presignis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.26
-
purified enzyme Cephaloleia presignis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O low activity Cephaloleia presignis 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O low activity Cephaloleia presignis 4-nitrophenol + butyrate
-
?
4-nitrophenyl laurate + H2O
-
Cephaloleia presignis 4-nitrophenol + laurate
-
?
4-nitrophenyl oleate + H2O
-
Cephaloleia presignis 4-nitrophenol + oleate
-
?
4-nitrophenyl palmitate + H2O high activity Cephaloleia presignis 4-nitrophenol + palmitate
-
?
4-nitrophenyl propionate + H2O
-
Cephaloleia presignis 4-nitrophenol + propionate
-
?
alpha-naphthyl acetate + H2O
-
Cephaloleia presignis alpha-naphthol + acetate
-
?
methyl acetate + H2O
-
Cephaloleia presignis methanol + acetate
-
?
methyl butyrate + H2O
-
Cephaloleia presignis methanol + butyrate
-
?
methyl laurate + H2O
-
Cephaloleia presignis methanol + laurate
-
?
methyl oleate + H2O high activity Cephaloleia presignis methanol + oleate
-
?
methyl palmitate + H2O high activity Cephaloleia presignis methanol + palmitate
-
?
methyl propionate + H2O
-
Cephaloleia presignis methanol + propionate
-
?
methyl stearate + H2O
-
Cephaloleia presignis methanol + stearate
-
?
additional information olive oil is utilized as substrate with high activity, the enzyme hydrolyzes long-chain fatty acids rather than short-chain fatty acids of 4-nitrophenyl esters and methyl esters, and it hydrolyzes short-chain fatty acids rather than short-shain fatty acids of triglycerides Cephaloleia presignis ?
-
?
tributyrin + H2O best substrate Cephaloleia presignis dibutyrin + butyrate
-
?
trilaurin + H2O high activity Cephaloleia presignis dilaurin + laurate
-
?
triolein + H2O
-
Cephaloleia presignis diolein + oleate
-
?
tripalmitin + H2O
-
Cephaloleia presignis dipalmitin + palmitate
-
?
tripropionin + H2O
-
Cephaloleia presignis dipropionin + propionate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Cephaloleia presignis

Synonyms

Synonyms Comment Organism
lipase
-
Cephaloleia presignis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Cephaloleia presignis

pI Value

Organism Comment pI Value Maximum pI Value
Cephaloleia presignis isoelectric focusing
-
6.9