Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Ca2+, Mn2+, Mg2+, Na+, K+, PMSF, EDTA, all at 1 mM | Cephaloleia presignis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition, 1 mM | Cephaloleia presignis | |
Cu2+ | complete inhibition, 1 mM | Cephaloleia presignis | |
diisopropyl fluorophosphate | complete inhibition, 1 mM | Cephaloleia presignis | |
Fe2+ | complete inhibition, 1 mM | Cephaloleia presignis | |
Fe3+ | complete inhibition, 1 mM | Cephaloleia presignis | |
additional information | not affected by Ca2+, Mn2+, Mg2+, Na+, K+, PMSF, EDTA, all at 1 mM | Cephaloleia presignis | |
Zn2+ | complete inhibition, 1 mM | Cephaloleia presignis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | hemolymph | Cephaloleia presignis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metalloenzyme | Cephaloleia presignis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Cephaloleia presignis |
31000 | - |
1 * 31000, SDS-PAGE | Cephaloleia presignis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cephaloleia presignis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cephaloleia presignis |
Purification (Comment) | Organism |
---|---|
20.3fold | Cephaloleia presignis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemolymph | - |
Cephaloleia presignis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.26 | - |
purified enzyme | Cephaloleia presignis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | low activity | Cephaloleia presignis | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | low activity | Cephaloleia presignis | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Cephaloleia presignis | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl oleate + H2O | - |
Cephaloleia presignis | 4-nitrophenol + oleate | - |
? | |
4-nitrophenyl palmitate + H2O | high activity | Cephaloleia presignis | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Cephaloleia presignis | 4-nitrophenol + propionate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Cephaloleia presignis | alpha-naphthol + acetate | - |
? | |
methyl acetate + H2O | - |
Cephaloleia presignis | methanol + acetate | - |
? | |
methyl butyrate + H2O | - |
Cephaloleia presignis | methanol + butyrate | - |
? | |
methyl laurate + H2O | - |
Cephaloleia presignis | methanol + laurate | - |
? | |
methyl oleate + H2O | high activity | Cephaloleia presignis | methanol + oleate | - |
? | |
methyl palmitate + H2O | high activity | Cephaloleia presignis | methanol + palmitate | - |
? | |
methyl propionate + H2O | - |
Cephaloleia presignis | methanol + propionate | - |
? | |
methyl stearate + H2O | - |
Cephaloleia presignis | methanol + stearate | - |
? | |
additional information | olive oil is utilized as substrate with high activity, the enzyme hydrolyzes long-chain fatty acids rather than short-chain fatty acids of 4-nitrophenyl esters and methyl esters, and it hydrolyzes short-chain fatty acids rather than short-shain fatty acids of triglycerides | Cephaloleia presignis | ? | - |
? | |
tributyrin + H2O | best substrate | Cephaloleia presignis | dibutyrin + butyrate | - |
? | |
trilaurin + H2O | high activity | Cephaloleia presignis | dilaurin + laurate | - |
? | |
triolein + H2O | - |
Cephaloleia presignis | diolein + oleate | - |
? | |
tripalmitin + H2O | - |
Cephaloleia presignis | dipalmitin + palmitate | - |
? | |
tripropionin + H2O | - |
Cephaloleia presignis | dipropionin + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE | Cephaloleia presignis |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Cephaloleia presignis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cephaloleia presignis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cephaloleia presignis | isoelectric focusing | - |
6.9 |