Literature summary for 3.1.1.29 extracted from

Burks, G.L.; McFeeters, H.; McFeeters, R.L.
Expression, purification, and buffer solubility optimization of the putative human peptidyl-tRNA hydrolase PTRHD1 (2016), Protein Expr. Purif., 126, 49-54 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene pth, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q6GMV3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by metal chelating affinity chromatography, dialysis, and ultrafiltration	Homo sapiens

Storage Stability

Storage Stability	Organism
PTRHD1 storage buffer is optimized in two stages using hanging microdrop screening, overview. The most favorable buffer for long-term storage and stability of PTRHD1 is 20 mM MES, 150 mM sodium chloride, pH 6.5. This buffer extends the soluble half-life at 4°C from days to many months for PTRHD1 concentrated to 0.5 mM or greater. The addition of other stabilizers (i.e. glycerol, PEG, etc.) is not pursued due to the tremendous increase in ability to store soluble PTRHD1 in solution	Homo sapiens

Organism

PTRHD1 storage buffer is optimized in two stages using hanging microdrop screening, overview. The most favorable buffer for long-term storage and stability of PTRHD1 is 20 mM MES, 150 mM sodium chloride, pH 6.5. This buffer extends the soluble half-life at 4°C from days to many months for PTRHD1 concentrated to 0.5 mM or greater. The addition of other stabilizers (i.e. glycerol, PEG, etc.) is not pursued due to the tremendous increase in ability to store soluble PTRHD1 in solution

Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	weak binding to peptidyl-tRNA for PTRHD1, binding to tRNA is detected but also the absence of peptidyl-tRNA hydrolase activity. Thus, PTRHD1 is not a Pth and the functional consequence of nucleotide binding remains undefined	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PTRHD1	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	performing the essential function of recycling peptidyl-tRNAs, peptidyl-tRNA hydrolases are ubiquitous in all domains of life. The multicomponent eukaryotic Pth system differs greatly from the bacterial system composed predominantly of a single Pth1 enzyme	Homo sapiens
additional information	PTRHD1 lacks the conserved, catalytically essential His20	Homo sapiens
physiological function	the enzyme is unable to complement a Pth1-deficient Salmonella typhimurium mutant strain. PTRHD1 does bind RNA but is not a peptidyl-tRNA hydrolase and its function needs to be determined for reclassification	Homo sapiens