KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of mutations altering the 1-72 pair of E. coli tRNAMetf on the Km-value | Escherichia coli | |
0.0022 | - |
N-acetyl-Met-tRNA | pH 7.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the enzyme almost completely inactive in absence of divalent cations. Mn2+ is partly effective, optimal concentration is about 0.2-0.5 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
construction of a bacterial strain overexpressing the enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-tRNAMet + H2O | - |
Escherichia coli | Met + tRNAMet | - |
? | |
additional information | derivatives of tRNAMetf with various combinations of bases at position 1 and 72 in the acceptor stem have been produced, aminoacylated and chemically acetylated. TrNAmetd derivatives with either C1A72, c1C72, U1G72, U1C72 or A1C72 behave as poor substrates of the enzyme compared to those with C1G72, U1A72, G1C72, A1U72 or G1U72 | Escherichia coli | ? | - |
? | |
N-acetyl-Met-tRNA + H2O | - |
Escherichia coli | N-acetyl-Met + tRNA | - |
? | |
Val-tRNAVal + H2O | - |
Escherichia coli | Val + tRNAVal | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of mutations altering the 1-72 pair of E. coli tRNAMetf on the turnover-number | Escherichia coli | |
0.7 | - |
N-acetyl-Met-tRNA | pH 7.0, 37°C | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
10 |