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Literature summary for 3.1.1.29 extracted from

  • de Pereda, J.M.; Waas, W.F.; Jan, Y.; Ruoslathi, E.; Schimmel, P.; Pascual, J.
    Crystral structure of a human peptidyl-tRNA hydrolase eveals a new fold and suggests basis for a bifunctional activity (2003), J. Biol. Chem., 279, 8111-8115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
full length human Pth2 clone as tempolate , subcloned into the NcoI/BamHI sites of the pET15b bacterial expression vector. The ligated plasmid is transfoirmed into Escherichia coli BL21(DE3) strain Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme, sitting drop vapor diffusion techniques Homo sapiens
sitting-drop vapor diffusion at room temperature, 10 mg/ml protein in 100 mM HEPES, pH 7.5 and 20% polyethylene glycol 10000 is equilibrated against a reservoir of the same solution, crystals diffract to 2.0 A Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000011
-
diacetyl-lysine-tRNA 27°C, pH 7.5 Saccharolobus solfataricus
0.00022
-
diacetyl-lysine-tRNA 27°C, pH 7.5 Homo sapiens
0.006
-
diacetyl-lysine-tRNA 27°C, pH 7.5 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-substituted aminoacyl-tRNA + H2O Escherichia coli
-
N-substituted amino acid + tRNA
-
?
N-substituted aminoacyl-tRNA + H2O Saccharolobus solfataricus
-
N-substituted amino acid + tRNA
-
?
N-substituted aminoacyl-tRNA + H2O Homo sapiens
-
N-substituted amino acid + tRNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens Q9Y3E5
-
-
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Pth Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacetyl-Lys-tRNA + H2O
-
Homo sapiens diacetyl-Lys + tRNA
-
?
diacetyl-Lys-tRNALys + H2O
-
Homo sapiens diacetyl-Lys + tRNA
-
?
diacetyl-lysine-tRNA + H2O
-
Escherichia coli diacetyl-lysine + tRNA
-
?
diacetyl-lysine-tRNA + H2O
-
Saccharolobus solfataricus diacetyl-lysine + tRNA
-
?
diacetyl-lysine-tRNA + H2O
-
Homo sapiens diacetyl-lysine + tRNA
-
?
N-substituted aminoacyl-tRNA + H2O
-
Escherichia coli N-substituted amino acid + tRNA
-
?
N-substituted aminoacyl-tRNA + H2O
-
Saccharolobus solfataricus N-substituted amino acid + tRNA
-
?
N-substituted aminoacyl-tRNA + H2O
-
Homo sapiens N-substituted amino acid + tRNA
-
?

Synonyms

Synonyms Comment Organism
PTH
-
Escherichia coli
Pth2
-
Saccharolobus solfataricus
Pth2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
diacetyl-Lys-tRNA pH 7.5, 27°C Homo sapiens
1.3
-
diacetyl-lysine-tRNA 27°C, pH 7.5 Homo sapiens
1.8
-
diacetyl-lysine-tRNA 27°C, pH 7.5 Saccharolobus solfataricus
3.6
-
diacetyl-lysine-tRNA 27°C, pH 7.5 Escherichia coli