Cloned (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM citrate (pH 5.8) and 100 mM tartrate | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
arachidonoyl-1-thio-glycerol | in 10 mM Tris-HCl (pH 7.2), temperature not specified in the publication | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | P82597 | - |
- |
Bacillus sp. (in: Bacteria) H-257 | P82597 | - |
- |
Purification (Comment) | Organism |
---|---|
octyl-Sepharose column chromatography, cation exchange column chromatography, and gel filtration | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-arachidonoylglycerol + H2O | - |
Bacillus sp. (in: Bacteria) | glycerol + arachidonic acid | - |
? | |
2-arachidonoylglycerol + H2O | - |
Bacillus sp. (in: Bacteria) H-257 | glycerol + arachidonic acid | - |
? | |
arachidonoyl-1-thio-glycerol + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
arachidonoyl-1-thio-glycerol + H2O | - |
Bacillus sp. (in: Bacteria) H-257 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MGL | - |
Bacillus sp. (in: Bacteria) |
monoacylglycerol lipase | - |
Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.66 | - |
arachidonoyl-1-thio-glycerol | in 10 mM Tris-HCl (pH 7.2), temperature not specified in the publication | Bacillus sp. (in: Bacteria) |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
arachidonoyl-1-thio-glycerol | in 10 mM Tris-HCl (pH 7.2), temperature not specified in the publication | Bacillus sp. (in: Bacteria) |