Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M MES/imidazole pH 6.5, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (w/v) MPD | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
JZL184 | - |
Bacillus sp. (in: Bacteria) | |
phenylmethylsulfonyl fluoride | - |
Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | P82597 | - |
- |
Bacillus sp. (in: Bacteria) H-257 | P82597 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
MGL | - |
Bacillus sp. (in: Bacteria) |
monoacylglycerol lipase | - |
Bacillus sp. (in: Bacteria) |