Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for design of potent and selective inhibitors | Homo sapiens |
pharmacology | the enzyme is a therapeutic target | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
functional overexpression of His6-tagged and streptavidin-tagged enzyme in Escherichia coli as soluble protein in the supernatant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-arachidonoylglycerol | - |
Homo sapiens | |
4-chloromercuribenzoic acid | - |
Homo sapiens | |
6-methyl-2-p-tolylamino-benzo[d] [1,3]oxazin-4-one | i.e. URB754 | Homo sapiens | |
arachidonoyltrifluoromethyl ketone | i.e. ATFMK | Homo sapiens | |
biphenyl-3-yl-carbamic acid cyclohexyl ester | i.e. URB602 | Homo sapiens | |
Disulfiram | i.e. tetraethylthiuram disulfide, a compound used to treat alcoholism, inhibition likely through an interaction with cysteine residues Cys208 and/or Cys242, the inhibition is reversible by DTT | Homo sapiens | |
Mercury chloride | - |
Homo sapiens | |
methyl arachidonyl fluorophosphonate | i.e. MAFP | Homo sapiens | |
additional information | no inhibition by URB754 | Homo sapiens | |
N-arachidonoylmaleimide | irreversible inhibition | Homo sapiens | |
N-ethylmaleimide | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | key enzyme responsible for the termination of endocannabinoid signaling with a crucial role in 2-arachidonoylglycerol metabolism, MAGL is unable to mediate anandamide degradation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged and streptavidin-tagged enzyme from Escherichia coli to homogeneity by Strep-tag targeting afffinity chromatography and nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oleoylglycerol + H2O | - |
Homo sapiens | glycerol + oleic acid | - |
? | |
additional information | key enzyme responsible for the termination of endocannabinoid signaling with a crucial role in 2-arachidonoylglycerol metabolism, MAGL is unable to mediate anandamide degradation, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAGL | - |
Homo sapiens |
monoacylglycerol lipase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
- |
Homo sapiens | additional information |