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Literature summary for 3.1.1.2 extracted from

  • Fenster, K.M.; Parkin, K.L.; Steele, J.L.
    Nucleotide sequencing, purification, and biochemical properties of an arylesterase from Lactobacillus casei LILA (2003), J. Dairy Sci., 86, 2547-2557.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl maximal activity at 35°C at 15% Lacticaseibacillus casei

Cloned(Commentary)

Cloned (Comment) Organism
gene estB, DNA and amino acid sequence determination and analysis, expression as His-tagged protein in Escherichia coli Lacticaseibacillus casei

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
Lacticaseibacillus casei
diisopropyl fluorophosphate
-
Lacticaseibacillus casei
iodoacetic acid slight inhibition Lacticaseibacillus casei
pepstatin A
-
Lacticaseibacillus casei
PMSF
-
Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
4-nitrophenyl hexanoate EstB, pH 7.5, 30°C Lacticaseibacillus casei
0.063
-
4-nitrophenyl pentanoate EstB, pH 7.5, 30°C Lacticaseibacillus casei
0.084
-
4-nitrophenyl decanoate EstB, pH 7.5, 30°C Lacticaseibacillus casei
0.23
-
4-nitrophenyl octanoate EstB, pH 7.5, 30°C Lacticaseibacillus casei
0.38
-
4-nitrophenyl butanoate EstB, pH 7.5, 30°C Lacticaseibacillus casei
1.4
-
4-nitrophenyl propionate EstB, pH 7.5, 30°C Lacticaseibacillus casei
1.9
-
4-nitrophenyl acetate EstB, pH 7.5, 30°C Lacticaseibacillus casei
3.2
-
ethyl hexanoate EstB, pH 7.5, 30°C Lacticaseibacillus casei
5.5
-
phenylthioacetate EstB, pH 7.5, 30°C Lacticaseibacillus casei
16
-
phenyl acetate EstB, pH 7.5, 30°C Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36700
-
6 * 36700, recombinant enzyme EstB, SDS-PAGE Lacticaseibacillus casei
216500
-
recombinant EstB, gel filtration Lacticaseibacillus casei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei Q7WZT5 LILA, enzyme EstB, gene estB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Lacticaseibacillus casei 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Lacticaseibacillus casei 4-nitrophenol + butyrate
-
?
4-nitrophenyl decanoate + H2O
-
Lacticaseibacillus casei 4-nitrophenol + decanoate
-
?
4-nitrophenyl hexanoate + H2O
-
Lacticaseibacillus casei 4-nitrophenol + hexanoate
-
?
4-nitrophenyl octanoate + H2O
-
Lacticaseibacillus casei 4-nitrophenol + octanoate
-
?
4-nitrophenyl pentanoate + H2O
-
Lacticaseibacillus casei 4-nitrophenol + pentanoate
-
?
4-nitrophenyl propionate + H2O
-
Lacticaseibacillus casei 4-nitrophenol + propionate
-
?
ethyl hexanoate + H2O
-
Lacticaseibacillus casei ethanol + hexanoate
-
?
additional information no activity with ethyl acetate, ethyl propionate, ethyl butanoate, ethyl pentanoate, propyl acetate, butyl acetate, hexanoyl acetate, and 4-nitrophenyl esters of chain length C10-C16 Lacticaseibacillus casei ?
-
?
phenyl acetate + H2O
-
Lacticaseibacillus casei phenol + acetate
-
?
phenylthioacetate + H2O
-
Lacticaseibacillus casei thiophenol + acetate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 36700, recombinant enzyme EstB, SDS-PAGE Lacticaseibacillus casei

Synonyms

Synonyms Comment Organism
EstB
-
Lacticaseibacillus casei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
Lacticaseibacillus casei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 deactivation Lacticaseibacillus casei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Lacticaseibacillus casei