Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | activates at lower concentration, Chlase2 reaches its maximal activity in 40% acetone, in 40-60% acetone the activity is reduced, overview | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
gene chl2, expression anaylsis, recombinant expression in Escherichia coli strain BL21 (DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | inhibition of expression of AtCLH2 by RNA interference, transfection using Agrobacterium tumefaciens strain LBA4404, AtCLH2 RNAi plants show decreased contents of chlorophyllide without a substantial change in the total amount of the extractable chlorophyll and consequently presented lower chlorophyllide to chlorophyll ratios in their leaves, phenotype, overview | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | activates at lower concentration, Chlase2 reaches its maximal activity in 40% acetone, in 40-60% acetone the activity is reduced, overview | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant AtCLH2, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | Arabidopsis thaliana | the enzyme is involved in the first step of chlorophyll degradation, molecular regulation of in vivo activities, AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo, overview | phytol + chlorophyllide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9M7I7 | isozyme AtCLH2; ecotype Columbia, isozyme AtCLH2 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme AtCLH2 from Escherichia coli strain BL21 (DE3) | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cauline leaf | - |
Arabidopsis thaliana | - |
flower | - |
Arabidopsis thaliana | - |
flower bud | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | spatial and temporal expression patterns of AtCLH2 in wild type plants, overview | Arabidopsis thaliana | - |
rosette leaf | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | - |
Arabidopsis thaliana | phytol + chlorophyllide | - |
? | |
chlorophyll + H2O | the enzyme is involved in the first step of chlorophyll degradation, molecular regulation of in vivo activities, AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo, overview | Arabidopsis thaliana | phytol + chlorophyllide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant AtCLH2, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtCLH2 | - |
Arabidopsis thaliana |
Chlase2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | isozyme AtCLH2 | Arabidopsis thaliana |