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Literature summary for 3.1.1.14 extracted from
- Optimization of chlorophyllase-catalyzed hydrolysis of chlorophyll in monophasic organic solvent media (2007), Appl. Biochem. Biotechnol., 142, 263-275.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | optimization of chlorophyllase-catalyzed hydrolysis of chlorophyll in monophasic organic solvent media, best in a mixture of hexane/2-octanone of 98.3:1.7 v/v, overview | Phaeodactylum tricornutum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast membrane | - |
Phaeodactylum tricornutum | 31969 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyll + H2O | Phaeodactylum tricornutum | the first enzyme in the chlorophyll degradation pathway in vivo catalyzes the hydrolysis of chlorophylls and pheophytins into their hydrophilic chromophore moieties chlorophyllides and pheophorbides, respectively | phytol + chlorophyllide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaeodactylum tricornutum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Phaeodactylum tricornutum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 322 | - |
optimization of chlorophyllase-catalyzed hydrolysis of chlorophyll in monophasic organic solvent media, the catalytic efficiency of chlorophyllase in the undiluted organic solvent mixture is lower than that in the aqueous medium, overview | Phaeodactylum tricornutum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyll + H2O | - |
Phaeodactylum tricornutum | phytol + chlorophyllide | - |
? | |
| chlorophyll + H2O | the first enzyme in the chlorophyll degradation pathway in vivo catalyzes the hydrolysis of chlorophylls and pheophytins into their hydrophilic chromophore moieties chlorophyllides and pheophorbides, respectively | Phaeodactylum tricornutum | phytol + chlorophyllide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chlorophyll-chlorophyllido-hydrolase | - |
Phaeodactylum tricornutum |
| More | the enzyme belongs to the alpha/beta-hydrolase fold family of enzymes | Phaeodactylum tricornutum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Phaeodactylum tricornutum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | - |
Phaeodactylum tricornutum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | enzyme half-life in the neat organic solvent compared to that in the aqueous medium in the first environment at temperature ranging from 35 to 50°C is increased by 5.0 to 15.0 times, overview | Phaeodactylum tricornutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Phaeodactylum tricornutum |
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Release 2023.1 (January 2023)
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