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Literature summary for 3.1.1.14 extracted from

  • Benedetti, C.E.; Arruda, P.
    Altering the expression of the chlorophyllase gene ATHCOR1 in transgenic Arabidopsis caused changes in the chlorophyll-to-chlorophyllide ratio (2002), Plant Physiol., 128, 1255-1263.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.14

Activating Compound

Activating Compound Comment Organism Structure
wounding induces enzyme expression from gene ATHCOR1 in leaves Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression of gene ATHCOR1 in Escherichia coli BL21 as maltose-binding protein fusion protein, cloning of the gene into an expression vector for transformation of Arabidopsis thaliana plants Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information gene ATHCOR1: construction of transgenic Arabidopsis thaliana plants overexpressing the enzyme via infection with Agrobacterium tumefaciens and transformation, sense and antisense orientation, the sense mutation changed the chlorophyll a to chlorophyll b ratio, overview, gene AtCLH2: cloning and analysis Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 strong inhibition at 0.2-1.0% Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
additional information divalent cations stimulate Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll a/b + H2O Arabidopsis thaliana the enzyme is involved in the regulation of chlorophyll a and b content phytol + chlorophyllide
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone strong reduction of activity at above 40% Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Columbia Col-0, gene ATHCOR1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding fusion protein from Escherichia coli Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
chlorophyll + H2O = phytol + chlorophyllide also catalyzes chlorophyllide transfer, e.g. converts chlorophyll to methylchlorophyllide Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
flower developing, constitutive expression of gene AtChl2 and gene ATHCOR1, expression of recmbinant enzyme in mutant transgenic plants Arabidopsis thaliana
-
flower bud AtChl2 Arabidopsis thaliana
-
leaf low content of ATHCOR1, expression of AtChl2 Arabidopsis thaliana
-
additional information not in root Arabidopsis thaliana
-

Storage Stability

Storage Stability Organism
4°C, stable Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll a/b + H2O specific for Arabidopsis thaliana phytol + chlorophyllide
-
 ?
chlorophyll a/b + H2O the enzyme is involved in the regulation of chlorophyll a and b content Arabidopsis thaliana phytol + chlorophyllide
-
 ?
additional information no activity with 4-nitrophenyl esters of fatty acids and tributyrin Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
Chlase
-
 Arabidopsis thaliana
coronatine-induced protein
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Arabidopsis thaliana