Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
food industry | fruit juice industry | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 52% residual activity at 100 mM | Aspergillus niger | |
K+ | 73% residual activity at 100 mM | Aspergillus niger | |
Mg2+ | 86% residual activity at 100 mM | Aspergillus niger | |
Mn2+ | 61% residual activity at 100 mM | Aspergillus niger | |
Ni2+ | 47% residual activity at 100 mM | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Na+ | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Aspergillus niger | - |
methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus niger |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HiTrap Q column chromatography, and Resource S column chromatography | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.67 | - |
supernatant, at pH 4.7 and 50°C | Aspergillus niger |
342 | - |
after 35.4fold purification, at pH 4.7 and 50°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Aspergillus niger | methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Aspergillus niger |
PME | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 90-100% activity from 35-50°C, about 30% activity at 55°C, no activity at 60°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | about 90% activity at pH 4.5,4.6, and 4.8, 100% activity at pH 4.7, about 60% activity at pH 4.9, about 50% activity at pH 5.0 | Aspergillus niger |