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Literature summary for 3.1.1.11 extracted from
- Pectin methylesterase activity and ascorbic acid content from guava fruit, cv. Predilecta, in different phases of development (2008), Int. J. Food Sci. Nutr., 60, 255-265.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | PME activity increases with fruit maturation | Psidium guajava | |
| NaCl | activity is highest in the presence of 0.3 M NaCl in 50 mM borate-acetate, pH 8.3 | Psidium guajava |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
guava PME contains two isoforms, one with 57000 Da molecular mass, SDS-PAGE | Psidium guajava |
| 99000 | - |
guava PME contains two isoforms, one with 99000 Da molecular mass, SDS-PAGE | Psidium guajava |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Psidium guajava | - |
cultivar Predilecta | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation | Psidium guajava |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | PME activity increases with fruit maturation | Psidium guajava | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | citrus pectin | Psidium guajava | methanol + pectate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectin methylesterase | - |
Psidium guajava |
| PME | - |
Psidium guajava |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Psidium guajava |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 98 | guava PME retains 96.8% of activity after 300 min in 90°C, retains 101.85% of its specific activity after 60 min of incubation at 50°C, the PME enzyme retains 75.4%, 86.2% and 90.4% of its specific activity after 60 min of incubation, respectively, at 80°C, 90°C, and 98°C | Psidium guajava |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Psidium guajava |
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Release 2023.1 (January 2023)
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