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Literature summary for 3.1.1.11 extracted from

  • Bosch, M.; Cheung, A.Y.; Hepler, P.K.
    Pectin methylesterase, a regulator of pollen tube growth (2005), Plant Physiol., 138, 1334-1346.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed as GFP-fusion protein in host strain, overexpression of PME results in reduced pollen tube growth Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Nicotiana tabacum 5783
-
Golgi stack
-
Nicotiana tabacum 5795
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Nicotiana tabacum
60300
-
x * 60300, calculated from the deduced amino acid sequence of the pre-pro-protein Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Nicotiana tabacum involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth methanol + pectate
-
?
pectin + H2O Lilium formosanum involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth methanol + pectate
-
?

Organism

Organism UniProt Comment Textmining
Lilium formosanum
-
cells cultured in the presence of PME from orange peel
-
Nicotiana tabacum
-
cells cultured in the presence of PME from orange peel
-

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
Nicotiana tabacum
-
pollen
-
Lilium formosanum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Nicotiana tabacum methanol + pectate
-
?
pectin + H2O
-
Lilium formosanum methanol + pectate
-
?
pectin + H2O involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth Nicotiana tabacum methanol + pectate
-
?
pectin + H2O involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth Lilium formosanum methanol + pectate
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Nicotiana tabacum
? x * 39000-41500, calculated from the deduced amino acid sequence for the mature protein Nicotiana tabacum
? x * 60300, calculated from the deduced amino acid sequence of the pre-pro-protein Nicotiana tabacum

Synonyms

Synonyms Comment Organism
Nicotiana tabacum pollen tube PME1 expression of the described pre-pro-PME is restricted to pollen and pollen tubes Nicotiana tabacum
NtPPME1
-
Nicotiana tabacum
pectin methylesterase
-
Nicotiana tabacum
pectin methylesterase
-
Lilium formosanum
PME
-
Nicotiana tabacum
PME
-
Lilium formosanum

pI Value

Organism Comment pI Value Maximum pI Value
Nicotiana tabacum isoelectric focusing
-
8.5
Nicotiana tabacum calculated from the deduced amino acid sequence for the mature protein
-
8.6