Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris as secretory protein | Ficus pumila var. awkeotsang |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE, protein from host | Ficus pumila var. awkeotsang |
45000 | - |
x * 45000, SDS-PAGE, recombinant protein | Ficus pumila var. awkeotsang |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Ficus pumila var. awkeotsang | involved in the regulation of the cell wall rigidity, key role in process of fruit ripening | methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ficus pumila var. awkeotsang | - |
jelly fig Makino | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | native protein has about 10% carbohydrates, recombinant protein appears to be hyperglycosylated | Ficus pumila var. awkeotsang |
Purification (Comment) | Organism |
---|---|
recombinant protein and native protein from host | Ficus pumila var. awkeotsang |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
achene | - |
Ficus pumila var. awkeotsang | - |
Storage Stability | Organism |
---|---|
4°C, fully stable for several months | Ficus pumila var. awkeotsang |
room temperature, fully stable for several days | Ficus pumila var. awkeotsang |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Ficus pumila var. awkeotsang | methanol + pectate | - |
? | |
pectin + H2O | involved in the regulation of the cell wall rigidity, key role in process of fruit ripening | Ficus pumila var. awkeotsang | methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE, protein from host | Ficus pumila var. awkeotsang |
? | x * 45000, SDS-PAGE, recombinant protein | Ficus pumila var. awkeotsang |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Ficus pumila var. awkeotsang |
PME | - |
Ficus pumila var. awkeotsang |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
no loss of activity within 2 h | Ficus pumila var. awkeotsang |
65 | - |
native enzyme stable for 30 min, 60% of activity remaining after 2 h | Ficus pumila var. awkeotsang |
70 | - |
20% of activity remaining after 2 h | Ficus pumila var. awkeotsang |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
native and recombinant protein | Ficus pumila var. awkeotsang |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | - |
Ficus pumila var. awkeotsang |