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Literature summary for 3.1.1.11 extracted from
- Pectinesterases from Phytophthora infestans (1988), Methods Enzymol., 161, 355-361.
No PubMed abstract available
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | CaCl2, stimulates, optimal concentration is 0.1 M | Phytophthora infestans |  |
| NaCl | stimulates, optimal concentration is 0.1 M | Phytophthora infestans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | 37000 | isoenzyme PE II, gel filtration | Phytophthora infestans |
| 40000 | - |
1 * 40000, isoenzyme PE II, SDS-PAGE | Phytophthora infestans |
| 45000 | - |
isoenzyme PE I, gel filtration | Phytophthora infestans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Phytophthora infestans | constitutive enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phytophthora infestans | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein | Phytophthora infestans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme PE I and isoenzyme PE II | Phytophthora infestans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Phytophthora infestans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Phytophthora infestans | methanol + pectate | - |
? | |
| pectin + H2O | constitutive enzyme | Phytophthora infestans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, isoenzyme PE II, SDS-PAGE | Phytophthora infestans |
| monomer | 1 * 45000-48000, isoenzyme PE I, SDS-PAGE | Phytophthora infestans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, inactivation | Phytophthora infestans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
isoenzyme PE I or PE II | Phytophthora infestans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH 4: 24% of maximal activity, pH 8: 98% of maximal activity, no activity at pH 9, isoenzyme PE II | Phytophthora infestans |
| 5 | 9 | pH 5: 58% of maximal activity, pH 9: 64% of maximal activity, isoenzyme PE I | Phytophthora infestans |
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