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Literature summary for 3.1.1.1 extracted from

  • Jackson, C.J.; Liu, J.W.; Carr, P.D.; Younus, F.; Coppin, C.; Meirelles, T.; Lethier, M.; Pandey, G.; Ollis, D.L.; Russell, R.J.; Weik, M.; Oakeshott, J.G.
    Structure and function of an insect alpha-carboxylesterase (alphaEsterase7) associated with insecticide resistance (2013), Proc. Natl. Acad. Sci. USA, 110, 10177-10182.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lucilia cuprina

Protein Variants

Protein Variants Comment Organism
M364L/I419F/A472T/I505T/K530E/D554G the mutant shows enhanced activity Lucilia cuprina

Organism

Organism UniProt Comment Textmining
Lucilia cuprina Q25252
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethyl-4-methylumbelliferyl phosphate + H2O
-
 Lucilia cuprina ?
-
 ?
methyl decanoate + H2O
-
 Lucilia cuprina methanol + decanoic acid
-
 ?
methyl hexanoate + H2O
-
 Lucilia cuprina methanol + hexanoic acid
-
 ?
methyl laurate + H2O
-
 Lucilia cuprina methanol + lauric acid
-
 ?
methyl myristate + H2O
-
 Lucilia cuprina methanol + myristic acid
-
 ?
methyl octanoate + H2O
-
 Lucilia cuprina methanol + octanoic acid
-
 ?

Subunits

Subunits Comment Organism
monomer or dimer
-
 Lucilia cuprina

Synonyms

Synonyms Comment Organism
alpha-carboxylesterase
-
 Lucilia cuprina
alphaE7
-
 Lucilia cuprina
alphaEsterase7
-
 Lucilia cuprina

General Information

General Information Comment Organism
physiological function the enzyme plays an important physiological role in lipid metabolism and is implicated in the detoxification of organophosphate insecticides Lucilia cuprina

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
50
-
 diethyl-4-methylumbelliferyl phosphate in 25 mM Tris-HCl (pH 8.0), at 25°C Lucilia cuprina
61
-
 methyl myristate in 25 mM Tris-HCl (pH 8.0), at 25°C Lucilia cuprina
200
-
 methyl laurate in 25 mM Tris-HCl (pH 8.0), at 25°C Lucilia cuprina
280
-
 methyl hexanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Lucilia cuprina
830
-
 methyl octanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Lucilia cuprina
1380
-
 methyl decanoate in 25 mM Tris-HCl (pH 8.0), at 25°C Lucilia cuprina