Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Haloarcula marismortui |
General Stability | Organism |
---|---|
the enzyme is totally unfolded in salt-free medium and secondary structure appears in the presence of 0.25–0.5 M KCl. After salt depletion, the protein is able to recover 60% of its initial activity when 2 M KCl is added | Haloarcula marismortui |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-methoxy-3-(4-phenoxyphenyl)-3H-[1,3,4]oxadiazol-2-one | half-inactivation time is 15 min, at an inhibitor:enzyme molar ratio of 100:1 | Haloarcula marismortui | |
E600 | half-inactivation times of 20 s, at an inhibitor:enzyme molar ratio is 10:1 | Haloarcula marismortui | |
phenylmethylsulfonyl fluoride | half-inactivation times is 10 s, at an inhibitor:enzyme molar ratio of 10:1 | Haloarcula marismortui |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activity is optimal in the presence of 3 M KCl and no activity is detected in the absence of salts. The enzyme is totally unfolded in salt-free medium and secondary structure appears in the presence of 0.25-0.5 M KCl. After salt depletion, the protein is able to recover 60% of its initial activity when 2 M KCl is added | Haloarcula marismortui |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula marismortui | Q5V5N6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula marismortui |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
590 | - |
pH 8.5, temperature not specified in the publication, substrate: vinyl butyrate | Haloarcula marismortui |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | specific activities on tri-, di- and monoacylglycerols are much lower than those measured with the shorter-chain vinyl esters and decreases with increasing acyl chain length. No detectable activity of the enzyme on vinyl esters and glycerol esters that have acyl chains longer than 12 carbon atoms | Haloarcula marismortui | ? | - |
? | |
vinyl acetate + H2O | 30.5% of the activity with vinyl butyrate | Haloarcula marismortui | vinyl alcohol + acetate | - |
? | |
vinyl butyrate + H2O | - |
Haloarcula marismortui | vinyl alcohol + butyrate | - |
? | |
vinyl hexanoate + H2O | 68% of the activity with vinyl butyrate | Haloarcula marismortui | vinyl alcohol + hexanoate | - |
? | |
vinyl propionate + H2O | 55% of the activity with vinyl butyrate | Haloarcula marismortui | vinyl alcohol + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LipC | - |
Haloarcula marismortui |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
2 h, no loss of activity | Haloarcula marismortui |
50 | - |
2 h, 4.5% loss of activity | Haloarcula marismortui |
60 | - |
5 min, complete loss of activity | Haloarcula marismortui |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
substrate: vinyl butyrate | Haloarcula marismortui |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | not active on vinyl butyrate at pH values below 5.0, above pH 5.0 the specific activity increases with pH, reaching a maximum of 590 U/mg at pH 8.5, pH 9.0: 78% of the maximum activity, substrate: vinyl butyrate | Haloarcula marismortui |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Haloarcula marismortui | calculated from sequence | - |
4.2 |