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Literature summary for 3.1.1.1 extracted from

  • Ikeda, M.; Clark, D.S.
    Molecular cloning of extremely thermostable esterase gene from hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli (1998), Biotechnol. Bioeng., 57, 624-629.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
 Pyrococcus furiosus 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl butyrate + H2O hydrolysis is 50% compared to 4-methylumbelliferyl acetate Pyrococcus furiosus 4-methylumbelliferone + butyrate
-
 ?
4-methylumbelliferyl palmitate + H2O hydrolysis is 7% compared to 4-methylumbelliferyl acetate Pyrococcus furiosus 4-methylumbelliferone + palmitate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Pyrococcus furiosus
100
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 110 80°C: about 55% of maximal activity, 110°C: about 80% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life: 34 h Pyrococcus furiosus
110
-
 half-life: 6 h Pyrococcus furiosus
120
-
 half-life: 2 h Pyrococcus furiosus
126
-
 half-life: 50 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.2 pH 7.0: about 40% of maximal activity, pH 8.2: about 50% of maximal activity Pyrococcus furiosus