Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorpyrifos-methyl | the enzyme is totally inhibited by 0.001 mM chlorpyrifos-methyl | Oryzaephilus surinamensis | |
chlorpyrifos-methyl oxon | the enzyme is totally inhibited by 0.001 mM chlorpyrifos-methyl oxon | Oryzaephilus surinamensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Oryzaephilus surinamensis |
120000 | - |
gel filtration | Oryzaephilus surinamensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryzaephilus surinamensis | - |
saw-toothed grain beetle, strains VOSCM and VOS48 | - |
Purification (Comment) | Organism |
---|---|
Sepharose CL-6B column chromatography, DEAE Sephacel gel filtration and Sephacryl S-300 gel filtration | Oryzaephilus surinamensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.77 | - |
crude extract from strain VOSCM, using 4-nitrophenyl acetate as substrate, in 9.2 mM Tris-HCl buffer, pH 7.5, at 25°C | Oryzaephilus surinamensis |
2 | 2.5 | enzyme from strain VOSCM after 29.2fold purification, using 4-nitrophenyl acetate as substrate, in 9.2 mM Tris-HCl buffer, pH 7.5, at 25°C | Oryzaephilus surinamensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Oryzaephilus surinamensis | 1-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Oryzaephilus surinamensis | 2-naphthol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | best substrate | Oryzaephilus surinamensis | 4-nitrophenol + acetate | - |
? | |
additional information | the purified enzyme does not hydrolyse insecticide substrates | Oryzaephilus surinamensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Oryzaephilus surinamensis |
General Information | Comment | Organism |
---|---|---|
physiological function | carboxylesterase from the chlorpyrifos-methyl-resistant strain VOSCM of Oryzaephilus surinamensis plays a role in conferring resistance to chlorpyrifos-methyl | Oryzaephilus surinamensis |