Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for the synthesis of 1-monocaprin, a widely used functional emulsifier in the food, cosmetic, and pharmaceutical industries and is recognized as a GRAS food additive | Calotropis procera |
Protein Variants | Comment | Organism |
---|---|---|
additional information | carboxylesterase is solubilized in reversed micellar glycerol droplets containing a very small amount of water and stabilized by a surfactant bis(2-ethylhexyl) sodium sulfosuccinate, optimization of carboxylesterase-catalyzed esterification between capric acid and glycerol for the production of 1-monocaprin in the reversed micellar system | Calotropis procera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 18% inhibition at 25 mM | Calotropis procera | |
Cu2+ | over 94% inhibition at 25 mM | Calotropis procera | |
Fe2+ | 97% inhibition at 25 mM | Calotropis procera | |
Sn2+ | 63% inhibition at 25 mM | Calotropis procera | |
Zn2+ | 46% inhibition at 25 mM | Calotropis procera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the carboxylesterase-catalyzed esterification of capric acid in the reversed micellar system follows Michaelis-Menten kinetics | Calotropis procera | |
9.64 | - |
capric acid | pH 10.0, 60°C | Calotropis procera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | latex | Calotropis procera | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Calotropis procera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially from latex by ammonium sulfate fractionation and dialysis | Calotropis procera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | from stems and leaves | Calotropis procera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
capric acid + sn-glycerol | the carboxylesterase performs also the esterification reaction using capric acid and glycerol as substrates resulting in formation of 1-monocaprin, product analysis by HPLC and method optimization, overview | Calotropis procera | 1-monocaprin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Calotropis procera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Calotropis procera |