Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.1 extracted from

  • Park, K.M.; Kwon, O.T.; Ahn, S.M.; Lee, J.; Chang, P.S.
    Characterization and optimization of carboxylesterase-catalyzed esterification between capric acid and glycerol for the production of 1-monocaprin in reversed micellar system (2010), New Biotechnol., 27, 46-52.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is useful for the synthesis of 1-monocaprin, a widely used functional emulsifier in the food, cosmetic, and pharmaceutical industries and is recognized as a GRAS food additive Calotropis procera

Protein Variants

Protein Variants Comment Organism
additional information carboxylesterase is solubilized in reversed micellar glycerol droplets containing a very small amount of water and stabilized by a surfactant bis(2-ethylhexyl) sodium sulfosuccinate, optimization of carboxylesterase-catalyzed esterification between capric acid and glycerol for the production of 1-monocaprin in the reversed micellar system Calotropis procera

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 18% inhibition at 25 mM Calotropis procera
Cu2+ over 94% inhibition at 25 mM Calotropis procera
Fe2+ 97% inhibition at 25 mM Calotropis procera
Sn2+ 63% inhibition at 25 mM Calotropis procera
Zn2+ 46% inhibition at 25 mM Calotropis procera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the carboxylesterase-catalyzed esterification of capric acid in the reversed micellar system follows Michaelis-Menten kinetics Calotropis procera
9.64
-
capric acid pH 10.0, 60°C Calotropis procera

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular latex Calotropis procera
-
-

Organism

Organism UniProt Comment Textmining
Calotropis procera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from latex by ammonium sulfate fractionation and dialysis Calotropis procera

Source Tissue

Source Tissue Comment Organism Textmining
latex from stems and leaves Calotropis procera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
capric acid + sn-glycerol the carboxylesterase performs also the esterification reaction using capric acid and glycerol as substrates resulting in formation of 1-monocaprin, product analysis by HPLC and method optimization, overview Calotropis procera 1-monocaprin
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Calotropis procera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Calotropis procera