Literature summary for 3.1.1.1 extracted from

Correia, M.A.; Prates, J.A.; Bras, J.; Fontes, C.M.; Newman, J.A.; Lewis, R.J.; Gilbert, H.J.; Flint, J.E.
Crystal structure of a cellulosomal family 3 carbohydrate esterase from Clostridium thermocellum provides insights into the mechanism of substrate recognition (2008), J. Mol. Biol., 379, 64-72.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Acetivibrio thermocellus

Protein Variants

Protein Variants	Comment	Organism
D205A	mutant shows decreased activity compared to the wild type enzyme	Acetivibrio thermocellus
D205N	mutant shows decreased activity compared to the wild type enzyme	Acetivibrio thermocellus
H208A	mutant displays no significant catalytic activity	Acetivibrio thermocellus
S44A	mutant displays no significant catalytic activity	Acetivibrio thermocellus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5	-	4-nitrophenyl acetate	mutant enzyme D205N	Acetivibrio thermocellus
1.8	-	4-nitrophenyl acetate	mutant enzyme D205A	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Acetivibrio thermocellus	4-nitrophenol + acetate	-	?
alpha-naphthyl acetate + H2O	-	Acetivibrio thermocellus	alpha-naphthol + acetate	-	?
additional information	the enzyme does not attack cephalosporin C, N-acetyl-glucosamine, acetyl deoxyadenosine, aryl-ferulates and aryl-coumorates	Acetivibrio thermocellus	?	-	?

Synonyms

Synonyms	Comment	Organism
Acetyl esterase	-	Acetivibrio thermocellus
Ces3-1	-	Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	74	isozymr Ces3-1 retains maximum catalytic activity when incubated for 15 min up to 70°C, after which the esterase is rapidly inactivated displaying no activity after treatment at 74°C for 15 min	Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.175	-	4-nitrophenyl acetate	mutant enzyme D205N	Acetivibrio thermocellus
0.665	-	4-nitrophenyl acetate	mutant enzyme D205A	Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Acetivibrio thermocellus

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Release 2023.1 (January 2023)