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Literature summary for 3.1.1.1 extracted from
- Cloning and characterization of thermostable esterase from Archaeoglobus fulgidus (2008), J. Microbiol., 46, 100-107.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
ketoprofen ethyl ester | - |
Archaeoglobus fulgidus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
gel filtration | Archaeoglobus fulgidus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Triton X-100 | 960% increase of activity in the presence of Triton X-100 | Archaeoglobus fulgidus |
| Triton X-15 | 110% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| Triton X-165 | 1649% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| Triton X-305 | 1528% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| Triton X-45 | 562% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| Tween | more than 1200% increase of activity in the presence of Tween 20, 40, 60 or 80 | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
strain DSM 4304 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | highest activity | Archaeoglobus fulgidus | 4-nitrophenol + butyrate | - |
? | |
| ketoprofen ethyl ester + H2O | - |
Archaeoglobus fulgidus | ketoprofen + ethanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27500, gel filtration | Archaeoglobus fulgidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Est-AF | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
| 80 | - |
using 4-nitrophenyl butyrate as substrate | Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 90 | more than 90% activity remains at 65°C or 70°C, 10% of activity remains after 3 h incubation at 90°C using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Archaeoglobus fulgidus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 12 | enzyme activity decreases above pH 8.0, no enzyme activity is observed at pH 3.0 and 12.0 | Archaeoglobus fulgidus |
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