Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.1 extracted from

  • Maqbool, Q.U.; Johri, S.; Rasool, S.; Riyaz-ul-Hassan, S.; Verma, V.; Nargotra, A.; Koul, S.; Qazi, G.N.
    Molecular cloning of carboxylesterase gene and biochemical characterization of encoded protein from Bacillus subtilis (RRL BB1) (2006), J. Biotechnol., 125, 1-10.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
alignment studies show that enzyme has an alpha/beta hydrolase fold with catalytic triad formed by S190, E305, and H394 Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, more than 70% loss of activity Bacillus subtilis
ascorbic acid 1 mM, 40% residual activity Bacillus subtilis
Cu2+ 1 mM, more than 70% loss of activity Bacillus subtilis
Fe2+ 1 mM, more than 70% loss of activity Bacillus subtilis
Fe3+ 1 mM, more than 70% loss of activity Bacillus subtilis
additional information not inhibitory: detergents CHAPS, Tween-20, Triton X-100 at 1%, or EDTA, dithiothreitol, 2-mercaptoethanol Bacillus subtilis
sodium dodecylsulfate 0.1%, strong inhibition Bacillus subtilis
Zn2+ 1 mM, more than 70% loss of activity Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
4-nitrophenyl butanoate pH 7.5, 22°C Bacillus subtilis
0.077
-
4-nitrophenyl caproate pH 7.5, 22°C Bacillus subtilis
0.196
-
Triacetin pH 7.5, 22°C Bacillus subtilis
2.22
-
tributyrin pH 7.5, 22°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
x * 52000, calculated Bacillus subtilis

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 20% v/v, 70-80% residual activity, 60% v/v, 45% residual activity Bacillus subtilis
Ethanol 20% v/v, 70-80% residual activity, 60% v/v, almost complete inactivation Bacillus subtilis
Methanol 20% v/v, 70-80% residual activity, 60% v/v, 45% residual activity Bacillus subtilis
propanol 20% v/v, 70-80% residual activity, 60% v/v, almost complete inactivation Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis Q4PNS3 enantioselective enzyme estBB1
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1735
-
pH 7.5, 22°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Bacillus subtilis 4-nitrophenol + acetate 92% of the activity with 4-nitrophenyl butanoate ?
4-nitrophenyl butanoate + H2O
-
Bacillus subtilis 4-nitrophenol + butanoate
-
?
4-nitrophenyl caproate + H2O
-
Bacillus subtilis 4-nitrophenol + caproate 65% of the activity with 4-nitrophenyl butanoate ?
4-nitrophenyl caprylate + H2O
-
Bacillus subtilis 4-nitrophenol + caprylate 45% of the activity with 4-nitrophenyl butanoate ?
4-nitrophenyl laurate + H2O
-
Bacillus subtilis 4-nitrophenol + laurate 30-35% of the activity with 4-nitrophenyl butanoate ?
ethyl 2-hydroxy-4-phenylbutanoate + H2O
-
Bacillus subtilis 2-hydroxy-4-phenylbutanoate + ethanol enantiopreference for (S)-isomer with enantiomeric excess of 80% ?
additional information no substrate: triglycerides with chain length of C8 to C18 Bacillus subtilis ?
-
?
triacetin + H2O about 65% of the activity with tributyrin Bacillus subtilis acetic acid + glycerol
-
?
tributyrin + H2O
-
Bacillus subtilis butanoate + glycerol
-
?

Subunits

Subunits Comment Organism
? x * 52000, calculated Bacillus subtilis

Synonyms

Synonyms Comment Organism
estBB1
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
sharp decrease in stability above Bacillus subtilis
50
-
30 min, complete loss of activity Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis calculated
-
5.2