Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
alignment studies show that enzyme has an alpha/beta hydrolase fold with catalytic triad formed by S190, E305, and H394 | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, more than 70% loss of activity | Bacillus subtilis | |
ascorbic acid | 1 mM, 40% residual activity | Bacillus subtilis | |
Cu2+ | 1 mM, more than 70% loss of activity | Bacillus subtilis | |
Fe2+ | 1 mM, more than 70% loss of activity | Bacillus subtilis | |
Fe3+ | 1 mM, more than 70% loss of activity | Bacillus subtilis | |
additional information | not inhibitory: detergents CHAPS, Tween-20, Triton X-100 at 1%, or EDTA, dithiothreitol, 2-mercaptoethanol | Bacillus subtilis | |
sodium dodecylsulfate | 0.1%, strong inhibition | Bacillus subtilis | |
Zn2+ | 1 mM, more than 70% loss of activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
4-nitrophenyl butanoate | pH 7.5, 22°C | Bacillus subtilis | |
0.077 | - |
4-nitrophenyl caproate | pH 7.5, 22°C | Bacillus subtilis | |
0.196 | - |
Triacetin | pH 7.5, 22°C | Bacillus subtilis | |
2.22 | - |
tributyrin | pH 7.5, 22°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, calculated | Bacillus subtilis |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 20% v/v, 70-80% residual activity, 60% v/v, 45% residual activity | Bacillus subtilis |
Ethanol | 20% v/v, 70-80% residual activity, 60% v/v, almost complete inactivation | Bacillus subtilis |
Methanol | 20% v/v, 70-80% residual activity, 60% v/v, 45% residual activity | Bacillus subtilis |
propanol | 20% v/v, 70-80% residual activity, 60% v/v, almost complete inactivation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q4PNS3 | enantioselective enzyme estBB1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1735 | - |
pH 7.5, 22°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | 92% of the activity with 4-nitrophenyl butanoate | ? | |
4-nitrophenyl butanoate + H2O | - |
Bacillus subtilis | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Bacillus subtilis | 4-nitrophenol + caproate | 65% of the activity with 4-nitrophenyl butanoate | ? | |
4-nitrophenyl caprylate + H2O | - |
Bacillus subtilis | 4-nitrophenol + caprylate | 45% of the activity with 4-nitrophenyl butanoate | ? | |
4-nitrophenyl laurate + H2O | - |
Bacillus subtilis | 4-nitrophenol + laurate | 30-35% of the activity with 4-nitrophenyl butanoate | ? | |
ethyl 2-hydroxy-4-phenylbutanoate + H2O | - |
Bacillus subtilis | 2-hydroxy-4-phenylbutanoate + ethanol | enantiopreference for (S)-isomer with enantiomeric excess of 80% | ? | |
additional information | no substrate: triglycerides with chain length of C8 to C18 | Bacillus subtilis | ? | - |
? | |
triacetin + H2O | about 65% of the activity with tributyrin | Bacillus subtilis | acetic acid + glycerol | - |
? | |
tributyrin + H2O | - |
Bacillus subtilis | butanoate + glycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, calculated | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
estBB1 | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
sharp decrease in stability above | Bacillus subtilis |
50 | - |
30 min, complete loss of activity | Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | calculated | - |
5.2 |