Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.1 extracted from

  • Morana, A.; Di Prizito, N.; Aurilia, V.; Rossi, M.; Cannio, R.
    A carboxylesterase from the hyperthermophilic archaeon Sulfolobus solfataricus: cloning of the gene, characterization of the protein. (2002), Gene, 283, 107-115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 1 mM, 35% inhibition Saccharolobus solfataricus
phenylmethylsulfonyl fluoride 1 mM, complete inhibition Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
4 * 32000, SDS-PAGE Saccharolobus solfataricus
33425
-
4 * 33425, calculated from sequence Saccharolobus solfataricus
114000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl acetate + H2O
-
Saccharolobus solfataricus 2-naphthol + acetate
-
?
2-naphthyl acetate + H2O
-
Saccharolobus solfataricus MT4 2-naphthol + acetate
-
?
4-nitrophenyl acetate + H2O at 37°C, 14% of the activity with pentanoyl-CoA Saccharolobus solfataricus 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O at 37°C, 14% of the activity with pentanoyl-CoA Saccharolobus solfataricus MT4 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O at 37°C, 74% of the activity with pentanoyl-CoA Saccharolobus solfataricus 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O at 37°C, 74% of the activity with pentanoyl-CoA Saccharolobus solfataricus MT4 4-nitrophenol + butyrate
-
?
4-nitrophenyl hexanoate + H2O at 37°C, 69% of the activity with pentanoyl-CoA Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
?
4-nitrophenyl pentanoate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + pentanoate
-
?
4-nitrophenyl pentanoate + H2O
-
Saccharolobus solfataricus MT4 4-nitrophenol + pentanoate
-
?
additional information no activity with triolein Saccharolobus solfataricus ?
-
?
additional information no activity with triolein Saccharolobus solfataricus MT4 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 32000, SDS-PAGE Saccharolobus solfataricus
tetramer 4 * 33425, calculated from sequence Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
24 h, stable Saccharolobus solfataricus
80
-
24 h, 50% loss of activity Saccharolobus solfataricus
90
-
24 h, 85% loss of activity Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Saccharolobus solfataricus