Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, 35% inhibition | Saccharolobus solfataricus | |
phenylmethylsulfonyl fluoride | 1 mM, complete inhibition | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Saccharolobus solfataricus |
33425 | - |
4 * 33425, calculated from sequence | Saccharolobus solfataricus |
114000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-naphthyl acetate + H2O | - |
Saccharolobus solfataricus | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Saccharolobus solfataricus MT4 | 2-naphthol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | at 37°C, 14% of the activity with pentanoyl-CoA | Saccharolobus solfataricus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | at 37°C, 14% of the activity with pentanoyl-CoA | Saccharolobus solfataricus MT4 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | at 37°C, 74% of the activity with pentanoyl-CoA | Saccharolobus solfataricus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | at 37°C, 74% of the activity with pentanoyl-CoA | Saccharolobus solfataricus MT4 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | at 37°C, 69% of the activity with pentanoyl-CoA | Saccharolobus solfataricus | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Saccharolobus solfataricus MT4 | 4-nitrophenol + pentanoate | - |
? | |
additional information | no activity with triolein | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity with triolein | Saccharolobus solfataricus MT4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Saccharolobus solfataricus |
tetramer | 4 * 33425, calculated from sequence | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
24 h, stable | Saccharolobus solfataricus |
80 | - |
24 h, 50% loss of activity | Saccharolobus solfataricus |
90 | - |
24 h, 85% loss of activity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Saccharolobus solfataricus |