Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
L362A | more sensitive to inhibition by organophosphates than wild-type | Bacillus subtilis |
L362D | more sensitive to inhibition by organophosphates than wild-type | Bacillus subtilis |
L362E | similar to mutant L362D, some decrease in kcat and Km value compared to wild-type | Bacillus subtilis |
L362R | 22fold decrease in ratio kcat/Km value, mutant is less sensitive to organophosphates | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methyl paraoxon | - |
Bacillus subtilis | |
paraoxon | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
trans-permethrin | mutant L362R, 37°C, pH 7.4 | Bacillus subtilis | |
0.013 | - |
trans-permethrin | mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | |
0.014 | - |
trans-permethrin | mutant L362D, 37°C, pH 7.4 | Bacillus subtilis | |
0.014 | - |
trans-permethrin | wild-type, 37°C, pH 7.4 | Bacillus subtilis | |
0.1 | - |
4-nitrophenyl valerate | mutant L362D, 37°C, pH 7.4 | Bacillus subtilis | |
0.15 | - |
4-nitrophenyl valerate | wild-type, 37°C, pH 7.4 | Bacillus subtilis | |
0.23 | - |
4-yethylumbelliferyl acetate | mutant L362D, 37°C, pH 7.4 | Bacillus subtilis | |
0.26 | - |
4-nitrophenyl valerate | mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | |
0.29 | - |
4-yethylumbelliferyl acetate | wild-type, 37°C, pH 7.4 | Bacillus subtilis | |
0.36 | - |
4-nitrophenyl valerate | mutant L362R, 37°C, pH 7.4 | Bacillus subtilis | |
0.43 | - |
4-yethylumbelliferyl acetate | mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | |
0.57 | - |
4-yethylumbelliferyl acetate | mutant L362R, 37°C, pH 7.4 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl acetate + H2O | - |
Bacillus subtilis | 4-methylumbelliferone + acetate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Bacillus subtilis | 4-nitrophenol + valerate | - |
? | |
trans-permethrin + H2O | - |
Bacillus subtilis | (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE and MALDI-TOF | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
trans-permethrin | mutant L362R, 37°C, pH 7.4 | Bacillus subtilis | |
2.1 | - |
trans-permethrin | mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | |
2.2 | - |
trans-permethrin | mutant L362E, 37°C, pH 7.4 | Bacillus subtilis | |
2.9 | - |
trans-permethrin | wild-type, 37°C, pH 7.4 | Bacillus subtilis | |
57 | - |
4-yethylumbelliferyl acetate | mutant L362R, 37°C, pH 7.4 | Bacillus subtilis | |
69 | - |
4-nitrophenyl valerate | mutant L362R, 37°C, pH 7.4 | Bacillus subtilis | |
110 | - |
4-yethylumbelliferyl acetate | mutant L362E, 37°C, pH 7.4 | Bacillus subtilis | |
140 | - |
4-yethylumbelliferyl acetate | wild-type, 37°C, pH 7.4 | Bacillus subtilis | |
150 | - |
4-yethylumbelliferyl acetate | mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | |
270 | - |
4-nitrophenyl valerate | mutant L362E, 37°C, pH 7.4 | Bacillus subtilis | |
300 | - |
4-nitrophenyl valerate | mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | |
580 | - |
4-nitrophenyl valerate | wild-type, 37°C, pH 7.4 | Bacillus subtilis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000008 | - |
mutant L362D, 37°C, pH 7.4 | Bacillus subtilis | paraoxon | |
0.000001 | - |
mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | paraoxon | |
0.0000013 | - |
wild-type, 37°C, pH 7.4 | Bacillus subtilis | paraoxon | |
0.0000031 | - |
mutant L362R, 37°C, pH 7.4 | Bacillus subtilis | paraoxon | |
0.000018 | - |
mutant L362A, 37°C, pH 7.4 | Bacillus subtilis | methyl paraoxon | |
0.000024 | - |
mutant L362D, 37°C, pH 7.4 | Bacillus subtilis | methyl paraoxon | |
0.000081 | - |
wild-type, 37°C, pH 7.4 | Bacillus subtilis | methyl paraoxon | |
0.00027 | - |
mutant L362R, 37°C, pH 7.4 | Bacillus subtilis | methyl paraoxon |