Protein Variants | Comment | Organism |
---|---|---|
E217M | mutant in anionic site, p1 subsite, pyrethroid hydrolysis similar to wild-type | Lucilia cuprina |
F309L | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis-substrate, strong increase for trans-substrate | Lucilia cuprina |
F354L | mutant in anionic site, p1 subsite, pyrethroid hydrolysis similar to wild-type | Lucilia cuprina |
F354W | mutant in anionic site, p1 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate | Lucilia cuprina |
G137D | mutant in oxyanion hole, marked decrease in pyrethroid hydrolysis | Lucilia cuprina |
G137D | strong decrease in pyrethroid hydrolysis | Drosophila melanogaster |
G137E | mutant in oxyanion hole, strong decrease in pyrethroid hydrolysis | Lucilia cuprina |
G137H | mutant in oxyanion hole, marked decrease in pyrethroid hydrolysis | Lucilia cuprina |
G137R | mutant in oxyanion hole, pyrethroid hydrolysis similar to wild-type | Lucilia cuprina |
W251A | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate | Lucilia cuprina |
W251G | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate | Lucilia cuprina |
W251L | marked increase in pyrethroid hydrolysis, trans:cis ratio for preference of substrate is 2:1 compared to 24:1 in wild-type | Drosophila melanogaster |
W251L | mutant in acyl pocket p2 subsite, strong increase in pyrethroid hydrolysis both for cis- and trans-substrate. Trans:cis ratio for preference of substrate is 2:1 compared to 27:1 in wild-type | Lucilia cuprina |
W251L/F309L | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate. Trans:cis ratio for preference of substrate is 2:1 compared to 27:1 in wild-type | Lucilia cuprina |
W251L/G137D | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate. Trans:cis ratio for preference of substrate is 2:1 compared to 27:1 in wild-type | Lucilia cuprina |
W251L/P250S | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate. Trans:cis ratio for preference of substrate is 3:1 compared to 27:1 in wild-type | Lucilia cuprina |
W251S | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate | Lucilia cuprina |
W251T | mutant in acyl pocket p2 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate | Lucilia cuprina |
Y148F | mutant in anionic site, p1 subsite, marked increase in pyrethroid hydrolysis both for cis- and trans-substrate | Lucilia cuprina |
Y148F/G137D | marked decrease in pyrethroid hydrolysis, trans:cis ratio for preference of substrate is 87:1 compared to 24:1 in wild-type | Lucilia cuprina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
expression in baculoviral system | - |
Lucilia cuprina | - |
expression in baculoviral system | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phenoxybenzyl (1R,3R)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1R-cis-NRDC157 | Drosophila melanogaster | (1R,3R)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? | |
3-phenoxybenzyl (1R,3R)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1R-cis-NRDC157 | Lucilia cuprina | (1R,3R)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? | |
3-phenoxybenzyl (1R,3S)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1R-trans-permethrin, strong chiral preference for the 1S over the 1R isomers of permethrin | Drosophila melanogaster | (1R,3S)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? | |
3-phenoxybenzyl (1R,3S)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1R-trans-permethrin, strong chiral preference for the 1S over the 1R isomers of permethrin | Lucilia cuprina | (1R,3S)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? | |
3-phenoxybenzyl (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1S-trans-permethrin, strong chiral preference for the 1S over the 1R isomers of permethrin | Drosophila melanogaster | (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? | |
3-phenoxybenzyl (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1S-trans-permethrin, strong chiral preference for the 1S over the 1R isomers of permethrin | Lucilia cuprina | (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? | |
3-phenoxybenzyl (1S,3S)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1S-cis-NRDC157 | Drosophila melanogaster | (1S,3S)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? | |
3-phenoxybenzyl (1S,3S)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O | i.e. 1S-cis-NRDC157 | Lucilia cuprina | (1S,3S)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol | - |
? |