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Literature summary for 3.1.1.1 extracted from
- Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family (2004), Eur. J. Biochem., 271, 3953-3961.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl 4-nitrophenyl phosphate | i.e. E600, strong inhibitor | Mycobacterium tuberculosis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31700 | - |
x * 31700, calculated | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
protein Rv1399c, overexpressed in Escherichia coli | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a carboxylic ester + H2O = an alcohol + a carboxylate | catalytic triad consists of S162, H290, and D260 and is located at the bottom of a solvent accessible pocket. The guanidine moiety close to H290 confers a low pH shift of the catalytic His molecule | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 184 | - |
substrate triacetin, pH 7.0, 25°C | Mycobacterium tuberculosis |
| 456 | - |
substrate tributyrine, pH 7.0, 25°C | Mycobacterium tuberculosis |
| 610 | - |
substrate vinyl acetate, pH 7.0, 25°C | Mycobacterium tuberculosis |
| 1123 | - |
substrate vinyl butanoate, pH 7.0, 25°C | Mycobacterium tuberculosis |
| 1350 | - |
substrate tripropionin, pH 7.0, 25°C | Mycobacterium tuberculosis |
| 1618 | - |
substrate vinyl propionate, pH 7.0, 25°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| triacetin + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
| tributyrin + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
| tripropionin + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
| vinyl acetate + H2O | - |
Mycobacterium tuberculosis | vinyl alcohol + acetate | - |
? | |
| vinyl butanoate + H2O | - |
Mycobacterium tuberculosis | vinyl alcohol + butanoate | - |
? | |
| vinyl propionate + H2O | - |
Mycobacterium tuberculosis | vinyl alcohol + propionate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31700, calculated | Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
no enzymic activity above | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | broad | Mycobacterium tuberculosis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000003 | - |
diethyl 4-nitrophenyl phosphate | pH 7.0, 25°C | Mycobacterium tuberculosis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mycobacterium tuberculosis | calculated | - |
4.53 |
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