Application | Comment | Organism |
---|---|---|
biotechnology | enzyme is an important biocatalyst for the preparation of a wide range of selectively protected and enantiomerically pure compounds | Aspergillus nomiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by EDTA, Ca2+, Mg2+, Co2+, Fe3+, Zn2+ | Aspergillus nomiae | |
PMSF | complete inhibition | Aspergillus nomiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Aspergillus nomiae | |
0.034 | - |
4-nitrophenyl valerate | pH 7.0, 37°C | Aspergillus nomiae | |
0.06 | - |
4-nitrophenyl butyrate | pH 7.0, 37°C | Aspergillus nomiae | |
0.075 | - |
n-butyl benzoate | pH 7.0, 37°C | Aspergillus nomiae | |
0.11 | - |
ethyl benzoate | pH 7.0, 37°C | Aspergillus nomiae | |
0.33 | - |
4-nitrophenyl caproate | pH 7.0, 37°C | Aspergillus nomiae | |
0.35 | - |
4-nitrophenyl propionate | pH 7.0, 37°C | Aspergillus nomiae | |
0.39 | - |
ethyl 4-hydroxybenzoate | pH 7.0, 37°C | Aspergillus nomiae | |
0.66 | - |
ethylene glycol dibenzoate | pH 7.0, 37°C | Aspergillus nomiae | |
1.2 | - |
ethyl 3-hydroxybenzoate | pH 7.0, 37°C | Aspergillus nomiae | |
1.3 | - |
4-nitrophenyl acetate | pH 7.0, 37°C | Aspergillus nomiae | |
2.3 | - |
ethyl 3-methoxybenzoate | pH 7.0, 37°C | Aspergillus nomiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion to the medium | Aspergillus nomiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by EDTA, Ca2+, Mg2+, Co2+, Fe3+, Zn2+ | Aspergillus nomiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Aspergillus nomiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nomiae | - |
- |
- |
Aspergillus nomiae HS-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from culture medium to homogeneity, 28fold | Aspergillus nomiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
135 | - |
purified enzyme, substrate ethyl benzoate | Aspergillus nomiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Aspergillus nomiae | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Aspergillus nomiae HS-1 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Aspergillus nomiae | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Aspergillus nomiae HS-1 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Aspergillus nomiae | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Aspergillus nomiae | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Aspergillus nomiae HS-1 | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Aspergillus nomiae | 4-nitrophenol + valerate | - |
? | |
ethyl 3-hydroxybenzoate + H2O | - |
Aspergillus nomiae | ethanol + 3-hydroxybenzoate | - |
? | |
ethyl 3-methoxybenzoate + H2O | - |
Aspergillus nomiae | ethanol + 3-methoxybenzoate | - |
? | |
ethyl 4-hydroxybenzoate + H2O | - |
Aspergillus nomiae | ethanol + 4-hydroxybenzoate | - |
? | |
ethyl 4-methoxybenzoate + H2O | - |
Aspergillus nomiae | ethanol + 4-methoxybenzoate | - |
? | |
ethyl benzoate + H2O | - |
Aspergillus nomiae | ethanol + benzoate | - |
? | |
ethylene glycol dibenzoate + H2O | - |
Aspergillus nomiae | ? | - |
? | |
ethylene glycol dibenzoate + H2O | - |
Aspergillus nomiae HS-1 | ? | - |
? | |
additional information | dramatic decline in activity with substrates of chain length above C6, no activity with ethyl esters of 2-hydroxy- and 2-methoxybenzoates, phenyl acetate, ferulate, and diethyl esters of terephthalate and phthalate | Aspergillus nomiae | ? | - |
? | |
additional information | dramatic decline in activity with substrates of chain length above C6, no activity with ethyl esters of 2-hydroxy- and 2-methoxybenzoates, phenyl acetate, ferulate, and diethyl esters of terephthalate and phthalate | Aspergillus nomiae HS-1 | ? | - |
? | |
n-butyl benzoate + H2O | - |
Aspergillus nomiae | n-butanol + benzoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Aspergillus nomiae |
Synonyms | Comment | Organism |
---|---|---|
carboxyl esterase | - |
Aspergillus nomiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus nomiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, pH 7.0, loss of 5% activity | Aspergillus nomiae |
50 | - |
15 min, pH 7.0, loss of 55% activity | Aspergillus nomiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Aspergillus nomiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | 80% activity at pH 4.0 and pH 9.0 | Aspergillus nomiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus nomiae | isoelectric focusing | - |
4.8 |