Cloned (Comment) | Organism |
---|---|
functional heterologous expression of the enzyme in Pichia pastoris was only possible after elimination of the C-terminal endoplasmic reticulum retention signal sequence His-Ala-Glu-Leu, the recombinant enzyme is secreted into the medium | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
PLE | - |
Sus scrofa PLE | - |
PLE | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a carboxylic ester + H2O = an alcohol + a carboxylate | active site residues are Ser203, His448, and Asp97 | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
600 | - |
recombinant enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Sus scrofa | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Sus scrofa PLE | 4-nitrophenol + acetate | - |
? | |
proline-beta-naphthylamide + H2O | - |
Sus scrofa | ? | - |
? | |
proline-beta-naphthylamide + H2O | - |
Sus scrofa PLE | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 61000-62000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
pig liver esterase | - |
Sus scrofa |
PLE | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sus scrofa |