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Literature summary for 3.1.1.1 extracted from

  • Zhang, J.; Liu, J.; Zhou, J.; Ren, Y.; Dai, X.; Xiang, H.
    Thermostable esterase from Thermoanaerobacter tengcongensis: high-level expression, purification and characterization (2003), Biotechnol. Lett., 25, 1463-1467.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) Caldanaerobacter subterraneus subsp. tengcongensis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 45% reduced activity Caldanaerobacter subterraneus subsp. tengcongensis
deoxycholate 50% inhibition at 1% Caldanaerobacter subterraneus subsp. tengcongensis
additional information no inhibition by 1% Triton X-100 and !% CHAPS, not affected by Ca2+, Mg2+, Mn2+, Li+, Na+, K+, EDTA, and PMSF Caldanaerobacter subterraneus subsp. tengcongensis
SDS almost complete inhibition at 1% Caldanaerobacter subterraneus subsp. tengcongensis
Tween 20 almost complete inhibition at 1% Caldanaerobacter subterraneus subsp. tengcongensis
Zn2+ 59% reduced activity Caldanaerobacter subterraneus subsp. tengcongensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Ca2+, Mg2+, Mn2+, Li+, Na+, K+, and EDTA, enzyme is no metalloenzyme Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 recombinant enzyme, gel filtration Caldanaerobacter subterraneus subsp. tengcongensis

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis
-
 gene lipA encodes a thermostable esterase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli, to homogeneity Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O 4-nitrophenyl propionate and 4-nitrophenyl butyrate are the best substrates Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl dodecanoate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl laurate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + laurate
-
 ?
4-nitrophenyl palmitate + H2O low activity Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl propionate + H2O 4-nitrophenyl propionate and 4-nitrophenyl butyrate are the best substrates Caldanaerobacter subterraneus subsp. tengcongensis 4-nitrophenol + propionate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000, recombinant enzyme, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Caldanaerobacter subterraneus subsp. tengcongensis
75
-
 optimal growth temperature in vivo Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 h, remaining activity more than 80% Caldanaerobacter subterraneus subsp. tengcongensis
70
-
 1.5 h, 50% remaining activity Caldanaerobacter subterraneus subsp. tengcongensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Caldanaerobacter subterraneus subsp. tengcongensis

pH Range

pH Minimum pH Maximum Comment Organism
8 10
-
 Caldanaerobacter subterraneus subsp. tengcongensis