Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3) | Burkholderia gladioli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Burkholderia gladioli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia gladioli | Q9LAB8 | gene EstC, formerly Pseudomonas marginata | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Burkholderia gladioli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a carboxylic ester + H2O = an alcohol + a carboxylate | catalytic triad near the C-terminus includes His275 | Burkholderia gladioli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl butyrate + H2O | high activity | Burkholderia gladioli | 4-methylumbelliferol + butyrate | - |
? | |
7-acetoxy-3-(2-benzothiazolyl)-4-cyanocoumarin + H2O | low activity | Burkholderia gladioli | ? | - |
? | |
alpha-naphthyl acetate + H2O | - |
Burkholderia gladioli | alpha-naphthol + acetate | - |
? | |
alpha-naphthyl butyrate + H2O | - |
Burkholderia gladioli | alpha-naphthol + butyrate | - |
? | |
alpha-naphthyl nonanoate + H2O | low activity | Burkholderia gladioli | alpha-naphthol + nonanoate | - |
? | |
alpha-naphthyl propionate + H2O | best substrate | Burkholderia gladioli | 1-naphthol + propionate | - |
? | |
alpha-naphthyl valerate + H2O | - |
Burkholderia gladioli | alpha-naphthol + valerate | - |
? | |
beta-naphthyl butyrate + H2O | - |
Burkholderia gladioli | beta-naphthol + butyrate | - |
? | |
beta-naphthyl caproate + H2O | low activity | Burkholderia gladioli | beta-naphthol + caproate | - |
? | |
beta-naphthyl propionate + H2O | best substrate | Burkholderia gladioli | 2-naphthol + propionate | - |
? | |
beta-naphthyl valerate + H2O | - |
Burkholderia gladioli | beta-naphthol + valerate | - |
? | |
additional information | no activity with alpha-naphthol palmitate, substrate specificity, activity with other nitrophenyl esters, naphthol-AS esters, and HPTS esters, overview | Burkholderia gladioli | ? | - |
? | |
resorufine-butyrate + H2O | low activity | Burkholderia gladioli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Burkholderia gladioli |
Synonyms | Comment | Organism |
---|---|---|
EstC | - |
Burkholderia gladioli |