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Literature summary for 3.1.1.1 extracted from

  • Kim, Y.S.; Lee, H.B.; Choi, K.D.; Park, S.; Yoo, O.J.
    Cloning of Pseudomonas fluorescens carboxylesterase gene and characterization of its product expressed in Escherichia coli (1994), Biosci. Biotechnol. Biochem., 58, 111-116.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM83 Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 5 min, slight decrease in activity Pseudomonas fluorescens
PMSF
-
 Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 gel filtration Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from transformed Escherichia coli Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Pseudomonas fluorescens 1-naphthol + acetate
-
 ?
2-naphthyl acetate + H2O
-
 Pseudomonas fluorescens 2-naphthol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl propionate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + propionate
-
 ?
benzyl acetate + H2O
-
 Pseudomonas fluorescens benzyl alcohol + acetate
-
 ?
benzyl propionate + H2O
-
 Pseudomonas fluorescens benzyl alcohol + propionate
-
 ?
beta-phenethyl acetate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
methyl acetate + H2O
-
 Pseudomonas fluorescens methanol + acetate
-
 ?
methyl butyrate + H2O
-
 Pseudomonas fluorescens methanol + butyrate
-
 ?
methyl caprate + H2O
-
 Pseudomonas fluorescens methanol + decanoate
-
 ?
methyl hexanoate + H2O
-
 Pseudomonas fluorescens methanol + hexanoate
-
 ?
methyl octanoate + H2O
-
 Pseudomonas fluorescens methanol + octanoate
-
 ?
methyl propionate + H2O no activity Pseudomonas fluorescens methanol + propionate
-
 ?
phenyl acetate + H2O
-
 Pseudomonas fluorescens phenol + acetate
-
 ?
triacetin + H2O
-
 Pseudomonas fluorescens acetic acid + glycerol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Pseudomonas fluorescens