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Literature summary for 3.1.1.1 extracted from
- Purification and characterization of an intracellular carboxylesterase from Arthrobacter viscosus NRRL B-1973 (1999), Enzyme Microb. Technol., 24, 200-208.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiobis-(2-nitrobenzoic acid) | weak | Rhizobium viscosum |  |
| Hg2+ | HgCl2 | Rhizobium viscosum | |
| N-bromosuccinimide | - |
Rhizobium viscosum | |
| NEM | weak | Rhizobium viscosum | |
| PCMB | weak | Rhizobium viscosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0308 | - |
4-nitrophenyl acetate | - |
Rhizobium viscosum | |
| 0.0558 | - |
Glucose pentaacetate | - |
Rhizobium viscosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16700 | - |
nondenaturing PAGE | Rhizobium viscosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizobium viscosum | - |
- |
- |
| Rhizobium viscosum NRRL B-1973 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizobium viscosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 55.4 | - |
- |
Rhizobium viscosum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several weeks | Rhizobium viscosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Rhizobium viscosum | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Rhizobium viscosum NRRL B-1973 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | weak | Rhizobium viscosum | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Rhizobium viscosum | 4-nitrophenol + propionate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Rhizobium viscosum NRRL B-1973 | 4-nitrophenol + propionate | - |
? | |
| glucose pentaacetate + H2O | - |
Rhizobium viscosum | ? | - |
? | |
| glucose pentaacetate + H2O | - |
Rhizobium viscosum NRRL B-1973 | ? | - |
? | |
| ispopropenyl acetate + H2O | - |
Rhizobium viscosum | isopropenol + acetate | - |
? | |
| additional information | the enzyme removes acetyl residues from xanthan, alginate, glucose pentaacetate, cellobiose octaacetate | Rhizobium viscosum | ? | - |
? | |
| additional information | the enzyme removes acetyl residues from xanthan, alginate, glucose pentaacetate, cellobiose octaacetate | Rhizobium viscosum NRRL B-1973 | ? | - |
? | |
| naphthyl acetate + H2O | - |
Rhizobium viscosum | naphthol + acetate | - |
? | |
| naphthyl acetate + H2O | - |
Rhizobium viscosum NRRL B-1973 | naphthol + acetate | - |
? | |
| triacetin + H2O | - |
Rhizobium viscosum | acetic acid + glycerol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 16700, SDS-PAGE | Rhizobium viscosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Rhizobium viscosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
48 h, stable | Rhizobium viscosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Rhizobium viscosum |
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