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Literature summary for 2.8.3.18 extracted from
- A specialized citric acid cycle requiring succinyl-coenzyme A (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti (2008), J. Bacteriol., 190, 4933-4940.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a C-terminal hexahistidine-containing fusion peptide | Acetobacter aceti |
| expression in Escherichia coli | Acetobacter aceti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | substrate inhibition. Enzyme shows an increased substrate inhibition at a lower pH | Acetobacter aceti |  |
| dethiaacetyl-CoA | - |
Acetobacter aceti | |
| Sodium borohydride | - |
Acetobacter aceti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
acetyl-CoA | pH 8.0, 25°C | Acetobacter aceti | |
| 0.0221 | - |
succinyl-CoA | - |
Acetobacter aceti | |
| 0.0221 | - |
succinyl-CoA | pH 8.0, 25°C | Acetobacter aceti | |
| 0.0223 | - |
acetyl-CoA | - |
Acetobacter aceti | |
| 0.9 | - |
succinate | - |
Acetobacter aceti | |
| 0.9 | - |
succinate | pH 8.0, 25°C | Acetobacter aceti | |
| 29 | - |
acetate | pH 5.0, 25°C | Acetobacter aceti | |
| 51 | - |
acetate | pH 6.0, 25°C | Acetobacter aceti | |
| 70 | - |
acetate | - |
Acetobacter aceti | |
| 70 | - |
acetate | pH 8.0, 25°C | Acetobacter aceti | |
| 130 | - |
acetoacetate | - |
Acetobacter aceti | |
| 130 | - |
acetoacetate | pH 8.0, 25°C | Acetobacter aceti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55847 | - |
3 * 55847, ESI-MS, 3 * 55847, calculated, His-tagged recombinant protein | Acetobacter aceti |
| 160000 | - |
gel filtration, His-tagged recombinant protein | Acetobacter aceti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter aceti | - |
- |
- |
| Acetobacter aceti | B3EY95 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using an ammonium sulfate fractionation procedure and an immobilized-metal affinity step. An 8-liter culture yields 9.5 mg of purified protein | Acetobacter aceti |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| succinyl-CoA + acetate = acetyl-CoA + succinate | reaction displays ping-pong kinetics and a modified-enzyme mechanism | Acetobacter aceti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.053 | - |
strain 2002, reverse reaction, pH 8.0 | Acetobacter aceti |
| 0.083 | - |
strain 1023, reverse reaction, pH 8.0 | Acetobacter aceti |
| 0.114 | - |
strain 2002, forward reaction, pH 8.0 | Acetobacter aceti |
| 0.17 | - |
strain 1023, forward reaction, pH 8.0 | Acetobacter aceti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetate + succinyl-CoA | - |
Acetobacter aceti | succinate + acetyl-CoA | - |
r | |
| acetoacetate + succinyl-CoA | - |
Acetobacter aceti | succinate + acetoacetyl-CoA | - |
r | |
| acetyl-CoA + succinate | - |
Acetobacter aceti | succinyl-CoA + acetate | - |
r | |
| succinate + dethiaacetyl-CoA | - |
Acetobacter aceti | ? | - |
? | |
| succinyl-CoA + acetate | - |
Acetobacter aceti | acetyl-CoA + succinate | - |
r | |
| succinyl-CoA + acetoacetate | - |
Acetobacter aceti | acetoacetyl-CoA + succinate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 55847, ESI-MS, 3 * 55847, calculated, His-tagged recombinant protein | Acetobacter aceti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetate CoA-transferase | - |
Acetobacter aceti |
| SCACT | - |
Acetobacter aceti |
| succinyl-CoA:acetate CoA-transferase | - |
Acetobacter aceti |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Acetobacter aceti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.5 | - |
acetoacetate | - |
Acetobacter aceti | |
| 36.5 | - |
acetoacetate | pH 8.0, 25°C | Acetobacter aceti | |
| 49 | - |
acetate | pH 5.0, 25°C | Acetobacter aceti | |
| 70.9 | - |
succinate | - |
Acetobacter aceti | |
| 70.9 | - |
succinate | pH 8.0, 25°C | Acetobacter aceti | |
| 75.2 | - |
acetyl-CoA | - |
Acetobacter aceti | |
| 75.2 | - |
acetyl-CoA | pH 8.0, 25°C | Acetobacter aceti | |
| 147 | - |
acetate | pH 6.0, 25°C | Acetobacter aceti | |
| 201 | - |
succinyl-CoA | - |
Acetobacter aceti | |
| 201 | - |
succinyl-CoA | pH 8.0, 25°C | Acetobacter aceti | |
| 280 | - |
acetate | - |
Acetobacter aceti | |
| 280 | - |
acetate | pH 8.0, 25°C | Acetobacter aceti | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Acetobacter aceti |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
dethiaacetyl-CoA | - |
Acetobacter aceti | |
| 0.0166 | - |
dethiaacetyl-CoA | pH 8.0, 25°C | Acetobacter aceti | |
| 500 | - |
acetate | pH 5.0, 25°C | Acetobacter aceti | |
| 500 | - |
acetate | pH: 5.0, shows an increased substrate inhibition at a lower pH | Acetobacter aceti | |
| 1100 | - |
acetate | pH 6.0, 25°C | Acetobacter aceti | |
| 1100 | - |
acetate | pH: 6.0, shows an increased substrate inhibition at a lower pH | Acetobacter aceti | |
| 1600 | - |
acetate | pH 8.0, 25°C | Acetobacter aceti | |
| 1600 | - |
acetate | pH: 8.0 | Acetobacter aceti | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Acetobacter aceti | calculated | - |
6.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme AarC is succinyl-coenzyme A:acetate CoA-transferase, which replaces succinyl-CoA synthetase in a variant citric acid cycle. This bypass appears to reduce metabolic demand for free CoA, reliance upon nucleotide pools, and the likely effect of variable cytoplasmic pH upon citric acid cycle flux | Acetobacter aceti |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
acetoacetate | pH 8.0, 25°C | Acetobacter aceti | |
| 4 | - |
acetate | pH 8.0, 25°C | Acetobacter aceti | |
| 79 | - |
succinate | pH 8.0, 25°C | Acetobacter aceti | |
| 3400 | - |
acetyl-CoA | pH 8.0, 25°C | Acetobacter aceti | |
| 9000 | - |
succinyl-CoA | pH 8.0, 25°C | Acetobacter aceti | |
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