KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
glutaryl-CoA | pH 7.8, 30°C, cosubstrate adipate | Rattus norvegicus | |
0.07 | - |
adipyl-CoA | pH 7.8, 30°C, cosubstrate glutarate | Rattus norvegicus | |
0.11 | - |
adipyl-CoA | pH 7.8, 30°C, cosubstrate succinate | Rattus norvegicus | |
0.13 | - |
malonyl-CoA | pH 7.8, 30°C, cosubstrate glutarate | Rattus norvegicus | |
0.15 | - |
succinyl-CoA | pH 7.8, 30°C, cosubstrate glutarate | Rattus norvegicus | |
0.16 | - |
adipyl-CoA | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate | Rattus norvegicus | |
0.18 | - |
glutaryl-CoA | pH 7.8, 30°C, cosubstrate succinate | Rattus norvegicus | |
0.18 | - |
3-hydroxy-3-methylglutaryl-CoA | pH 7.8, 30°C, cosubstrate adipate, reverse reaction | Rattus norvegicus | |
0.18 | - |
Glutarate | pH 7.8, 30°C, cosubstrate adipylCoA | Rattus norvegicus | |
0.26 | - |
succinyl-CoA | pH 7.8, 30°C, cosubstrate adipate | Rattus norvegicus | |
0.26 | - |
Glutarate | pH 7.8, 30°C, cosubstrate malonylCoA | Rattus norvegicus | |
0.27 | - |
glutaryl-CoA | pH 7.8, 30°C, cosubstrate malonate | Rattus norvegicus | |
0.28 | - |
succinyl-CoA | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate | Rattus norvegicus | |
0.33 | - |
malonyl-CoA | pH 7.8, 30°C, cosubstrate adipate | Rattus norvegicus | |
0.38 | - |
adipyl-CoA | pH 7.8, 30°C, cosubstrate malonate | Rattus norvegicus | |
0.39 | - |
3-hydroxy-3-methylglutaryl-CoA | pH 7.8, 30°C, cosubstrate succinate or glutarate, reverse reaction | Rattus norvegicus | |
0.39 | - |
adipate | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA , reverse reaction | Rattus norvegicus | |
0.4 | - |
adipyl-CoA | pH 7.8, 30°C, cosubstrate methylmalonate | Rattus norvegicus | |
0.45 | - |
glutaryl-CoA | pH 7.8, 30°C, cosubstrate methylmalonate | Rattus norvegicus | |
0.45 | - |
Glutarate | pH 7.8, 30°C, cosubstrate succinylCoA | Rattus norvegicus | |
0.5 | - |
Glutarate | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA | Rattus norvegicus | |
0.5 | - |
succinate | pH 7.8, 30°C, cosubstrate glutarylCoA | Rattus norvegicus | |
0.52 | - |
malonyl-CoA | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate | Rattus norvegicus | |
0.55 | - |
malonyl-CoA | pH 7.8, 30°C, cosubstrate succinate | Rattus norvegicus | |
0.62 | - |
3-hydroxy-3-methylglutaryl-CoA | pH 7.8, 30°C, cosubstrate methylmalonate, reverse reaction | Rattus norvegicus | |
0.65 | - |
methylmalonyl-CoA | pH 7.8, 30°C, cosubstrate malonate | Rattus norvegicus | |
0.68 | - |
succinate | pH 7.8, 30°C, cosubstrate malonylCoA | Rattus norvegicus | |
0.7 | - |
malonyl-CoA | pH 7.8, 30°C, cosubstrate methylmalonate | Rattus norvegicus | |
0.72 | - |
adipate | pH 7.8, 30°C, cosubstrate glutaryl-CoA | Rattus norvegicus | |
0.72 | - |
methylmalonyl-CoA | pH 7.8, 30°C, cosubstrate glutarate or adipate | Rattus norvegicus | |
0.73 | - |
glutaryl-CoA | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate | Rattus norvegicus | |
0.74 | - |
methylmalonyl-CoA | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate | Rattus norvegicus | |
0.75 | - |
succinylCoA | pH 7.8, 30°C, cosubstrate malonate | Rattus norvegicus | |
0.76 | - |
3-hydroxy-3-methylglutarate | pH 7.8, 30°C, cosubstrate adipyl-CoA | Rattus norvegicus | |
0.83 | - |
adipate | pH 7.8, 30°C, cosubstrate malonylCoA | Rattus norvegicus | |
0.83 | - |
succinyl-CoA | pH 7.8, 30°C, cosubstrate methylmalonate | Rattus norvegicus | |
0.84 | - |
Glutarate | pH 7.8, 30°C, cosubstrate methylmalonylCoA | Rattus norvegicus | |
0.87 | - |
methylmalonyl-CoA | pH 7.8, 30°C, cosubstrate succinate | Rattus norvegicus | |
0.88 | - |
succinate | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA, reverse reaction | Rattus norvegicus | |
0.95 | - |
succinate | pH 7.8, 30°C, cosubstrate adipyl-CoA | Rattus norvegicus | |
1.01 | - |
malonate | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA , reverse reaction | Rattus norvegicus | |
1.2 | - |
succinate | pH 7.8, 30°C, cosubstrate methylmalonyl-CoA | Rattus norvegicus | |
1.25 | - |
3-hydroxy-3-methylglutarate | pH 7.8, 30°C, cosubstrate glutaryl-CoA | Rattus norvegicus | |
1.32 | - |
adipate | pH 7.8, 30°C, cosubstrate succinylCoA | Rattus norvegicus | |
1.42 | - |
3-hydroxy-3-methylglutarate | pH 7.8, 30°C, cosubstrate succinyl-CoA | Rattus norvegicus | |
1.51 | - |
adipate | pH 7.8, 30°C, cosubstrate methylmalonyl-CoA | Rattus norvegicus | |
1.55 | - |
malonate | pH 7.8, 30°C, cosubstrate glutaryl-CoA | Rattus norvegicus | |
1.63 | - |
malonate | pH 7.8, 30°C, cosubstrate succinyl-CoA or methylmalonyl-CoA | Rattus norvegicus | |
1.71 | - |
malonate | pH 7.8, 30°C, cosubstrate adipyl-CoA | Rattus norvegicus | |
1.8 | - |
3-hydroxy-3-methylglutarate | pH 7.8, 30°C, cosubstrate malonyl-CoA | Rattus norvegicus | |
1.95 | - |
methylmalonate | pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA, reverse reaction | Rattus norvegicus | |
2.2 | - |
methylmalonate | pH 7.8, 30°C, cosubstrate malonylCoA | Rattus norvegicus | |
2.33 | - |
3-hydroxy-3-methylglutarate | pH 7.8, 30°C, cosubstrate methylmalonyl-CoA | Rattus norvegicus | |
2.44 | - |
methylmalonate | pH 7.8, 30°C, cosubstrate glutaryl-CoA | Rattus norvegicus | |
2.7 | - |
methylmalonate | pH 7.8, 30°C, cosubstrate succinyl-CoA | Rattus norvegicus | |
3.05 | - |
methylmalonate | pH 7.8, 30°C, cosubstrate adipyl-CoA | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + 3-hydroxy-3-methylglutarate | Rattus norvegicus | involved in regulation of the tricarboxylic acid cycle rate and ketogenesis | succinate + 3-hydroxy-3-methylglutaryl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + adipate | - |
Rattus norvegicus | 3-hydroxy-3-methylglutarate + adipyl-CoA | - |
r | |
3-hydroxy-3-methylglutaryl-CoA + glutarate | - |
Rattus norvegicus | 3-hydroxy-3-methylglutarate + glutaryl-CoA | - |
r | |
3-hydroxy-3-methylglutaryl-CoA + malonate | - |
Rattus norvegicus | 3-hydroxy-3-methylglutarate + malonyl-CoA | - |
r | |
3-hydroxy-3-methylglutaryl-CoA + methylmalonate | - |
Rattus norvegicus | 3-hydroxy-3-methylglutarate + methylmalonyl-CoA | - |
r | |
3-hydroxy-3-methylglutaryl-CoA + succinate | - |
Rattus norvegicus | 3-hydroxy-3-methylglutarate + succinyl-CoA | - |
r | |
adipyl-CoA + 3-hydroxy-3-methylglutarate | - |
Rattus norvegicus | adipate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
adipyl-CoA + 3-hydroxy-3-methylglutarate | adipyl-CoA shows highest apparent affinty, forward reaction at 64%, reverse reaction at 32% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | adipate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
adipyl-CoA + glutarate | forward reaction at 82%, reverse reaction at 68% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | adipate + glutaryl-CoA | - |
r | |
adipyl-CoA + malonate | forward reaction at 40%, reverse reaction at 43% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | adipate + malonyl-CoA | - |
r | |
adipyl-CoA + methylmalonate | poor substrate, succinyl-CoA, glutaryl-CoA, malonyl-CoA or 3-hydroxy-3-methylglutaryl-CoA can replace adipyl-CoA | Rattus norvegicus | adipate + methylmalonyl-CoA | - |
r | |
adipyl-CoA + succinate | best substrates, reverse reaction at 38% the rate of forward reaction | Rattus norvegicus | adipate + succinyl-CoA | - |
r | |
glutaryl-CoA + 3-hydroxy-3-methylglutarate | forward and reverse reaction at 50% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | glutarate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
glutaryl-CoA + adipate | - |
Rattus norvegicus | glutarate + adipyl-CoA | - |
r | |
glutaryl-CoA + malonate | forward reaction at 33%, reverse reaction at 41% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | glutarate + malonyl-CoA | - |
r | |
glutaryl-CoA + methylmalonate | - |
Rattus norvegicus | glutarate + methylmalonyl-CoA | - |
r | |
glutaryl-CoA + succinate | - |
Rattus norvegicus | glutarate + succinyl-CoA | - |
r | |
malonyl-CoA + 3-hydroxy-3-methylglutarate | forward reaction at 34%, reverse reaction at 23% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | malonate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
malonyl-CoA + adipate | - |
Rattus norvegicus | malonate + adipyl-CoA | - |
r | |
malonyl-CoA + glutarate | - |
Rattus norvegicus | malonate + glutaryl-CoA | - |
r | |
malonyl-CoA + methylmalonate | - |
Rattus norvegicus | malonate + methylmalonyl-CoA | - |
r | |
malonyl-CoA + succinate | - |
Rattus norvegicus | malonate + succinyl-CoA | - |
r | |
methylmalonyl-CoA + 3-hydroxy-3-methylglutarate | - |
Rattus norvegicus | methylmalonate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
methylmalonyl-CoA + adipate | - |
Rattus norvegicus | methylmalonate + adipyl-CoA | - |
r | |
methylmalonyl-CoA + glutarate | - |
Rattus norvegicus | methylmalonate + glutaryl-CoA | - |
r | |
methylmalonyl-CoA + malonate | - |
Rattus norvegicus | methylmalonate + malonyl-CoA | - |
r | |
methylmalonyl-CoA + succinate | - |
Rattus norvegicus | methylmalonate + succinyl-CoA | - |
r | |
succinyl-CoA + 3-hydroxy-3-methylglutarate | - |
Rattus norvegicus | succinate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
succinyl-CoA + 3-hydroxy-3-methylglutarate | involved in regulation of the tricarboxylic acid cycle rate and ketogenesis | Rattus norvegicus | succinate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
succinyl-CoA + adipate | - |
Rattus norvegicus | succinate + adipyl-CoA | - |
r | |
succinyl-CoA + glutarate | forward reaction at 58%, reverse reaction at 55% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | succinate + glutaryl-CoA | - |
r | |
succinyl-CoA + malonate | forward reaction at 29%, reverse reaction at 22% the rate of the CoA-transfer between adipyl-CoA and succinate | Rattus norvegicus | succinate + malonyl-CoA | - |
r | |
succinyl-CoA + methylmalonate | - |
Rattus norvegicus | succinate + methylmalonyl-CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
dicarboxyl-CoA:dicarboxylic acid coenzyme A transferase | this seems to be a more appropriate name than the recommended or systematic name, due to the substrate specificity as documented | Rattus norvegicus |