Literature summary for 2.8.3.13 extracted from

Francesconi, M.A.; Donella-Deana, A.; Furlanetto, V.; Cavallini, L.; Palatini, P.; Deana, R.
Further purification and characterization of the succinyl-CoA:3-hydroxy-3-methylglutarate coenzyme A transferase from rat-liver mitochondria (1989), Biochim. Biophys. Acta, 999, 163-170.

Search for more literature for 2.8.3.13

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	not activated by 2-mercaptoethanol	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
acetate	-	Rattus norvegicus
acetoacetate	-	Rattus norvegicus
acetoacetyl-CoA	non-competitive inhibition	Rattus norvegicus
acetyl-CoA	non-competitive inhibition	Rattus norvegicus
butyryl-CoA	non-competitive inhibition	Rattus norvegicus
carnitine	-	Rattus norvegicus
Cl-	high concentration	Rattus norvegicus
ClO4-	high concentration	Rattus norvegicus
coenzyme A	non-competitive inhibition	Rattus norvegicus
F-	high concentration	Rattus norvegicus
I-	high concentration	Rattus norvegicus
additional information	only negligibly affected by sulfhydryl reagents and by the cations Li+, Cd2+, Ca2+, Mg2+, Mn2+ and Fe2+	Rattus norvegicus
succinate	competitive inhibition of the product	Rattus norvegicus
ZnCl2	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.25	-	succinyl-CoA	pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate	Rattus norvegicus
0.32	-	3-hydroxy-3-methylglutaryl-CoA	pH 7.8, 30°C, cosubstrate succinate or malonate, reverse reaction	Rattus norvegicus
0.73	-	succinate	pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA, reverse reaction	Rattus norvegicus
1.78	-	3-hydroxy-3-methylglutarate	pH 7.8, 30°C	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rattus norvegicus	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	52000	gel filtration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
succinyl-CoA + 3-hydroxy-3-methylglutarate	Rattus norvegicus	involved in regulation of the tricarboxylic acid cycle rate and ketogenesis	succinate + 3-hydroxy-3-methylglutaryl-CoA	-	r

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.248	-	-	Rattus norvegicus

Storage Stability

Storage Stability	Organism
-20°C, 0.1 M KH2PO4, pH 7.8, retains 70% of its activity after 2 months of storage	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-CoA + 3-hydroxy-3-methylglutarate	-	Rattus norvegicus	succinate + 3-hydroxy-3-methylglutaryl-CoA	-	r
succinyl-CoA + 3-hydroxy-3-methylglutarate	involved in regulation of the tricarboxylic acid cycle rate and ketogenesis	Rattus norvegicus	succinate + 3-hydroxy-3-methylglutaryl-CoA	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 12000-14000, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
succinylCoA:3-hydroxy-3-methylglutarate coenzyme A transferase	-	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Rattus norvegicus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)