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Literature summary for 2.8.2.9 extracted from

  • King, R.S.; Duffel, M.W.
    Oxidation-dependent inactivation of aryl sulfotransferase IV by primary N-hydroxy arylamines during in vitro assays (1997), Carcinogenesis, 18, 843-849.
    View publication on PubMed
Search for more literature for 2.8.2.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4-chloro-N-hydroxyaniline causes inhibition under reaction conditions where oxidative degradation occurs Rattus norvegicus
N-hydroxy-2-aminofluorene N-OH-2AF, causes inhibition under reaction conditions where oxidative degradation occurs Rattus norvegicus
N-hydroxyaniline causes inhibition under reaction conditions where oxidative degradation occurs, activity rapidly decreases to less than 10% of the initial activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 4-chloro-N-hydroxyaniline pH 7, 37°C Rattus norvegicus
0.23
-
 N-hydroxyaniline pH 7, 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
 x * 33500, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male
-
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.104
-
 recombinant enzyme Rattus norvegicus
0.8 1 native enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, stable for 6 months in sodium acetate buffer, pH 5.5, 0.25 M sucrose, 10% glycerol, 1 mM dithiothreitol at protein concentration of 1 mg/ml Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-chloro-N-hydroxyaniline oxidative degradation N-hydroxyaniline causes inhibition Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 4-chloro-N-hydroxyaniline oxidative degradation N-hydroxyaniline causes inhibition Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + N-hydroxy-2-aminofluorene causes inhibition under reaction conditions where oxidative degradation occurs Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + N-hydroxy-2-aminofluorene causes inhibition under reaction conditions where oxidative degradation occurs Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + N-hydroxyaniline oxidative degradation of N-hydroxyaniline causes inhibition Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + N-hydroxyaniline oxidative degradation of N-hydroxyaniline causes inhibition Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 ?
additional information forms sulfuric acid esters from a wide range of phenols, tyrosine carboxyesters, catechols, benzylic alcohols, arylhydroxamic acids and N-hydroxy arylamines. Stereochemical configuration of chiral benzyclic alcohols is an important factor in substrate specificity. One enantiomer may act as substrate, the other as inhibitor Rattus norvegicus ?
-
 ?
additional information forms sulfuric acid esters from a wide range of phenols, tyrosine carboxyesters, catechols, benzylic alcohols, arylhydroxamic acids and N-hydroxy arylamines. Stereochemical configuration of chiral benzyclic alcohols is an important factor in substrate specificity. One enantiomer may act as substrate, the other as inhibitor Rattus norvegicus Sprague-Dawley ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33500, SDS-PAGE Rattus norvegicus