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Literature summary for 2.8.2.5 extracted from

  • Yusa, A.; Kitajima, K.; Habuchi, O.
    N-linked oligosaccharides are required to produce and stabilize the active form of chondroitin 4-sulphotransferase-1 (2005), Biochem. J., 388, 115-121.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.5

Protein Variants

Protein Variants Comment Organism
additional information deletion of N-glycosylation sites, loss of the N-glycosylation site at the C-terminus reduces activity by 80% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 3'-phosphoadenylylsulfate pH 6.8, 37°C, wild-type Rattus norvegicus
0.0011
-
 3'-phosphoadenylylsulfate pH 6.8, 37°C, mutant with deletion of C-terminal glycosylation site Rattus norvegicus
2.1
-
 chondroitin pH 6.8, 37°C, wild-type Rattus norvegicus
2.7
-
 chondroitin pH 6.8, 37°C, mutant with deletion of C-terminal glycosylation site Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains about 35% N-linked oligosaccharides, treatment of enzyme with peptide N-glycosidase F causes a marked decrease in activity, production of active form of recombinant enzyme is inhibited by tunicamycin Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
chondrosarcoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + chondroitin
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?