Literature summary for 2.8.2.20 extracted from

Goettsch, S.; Badea, R.A.; Mueller, J.W.; Wotzlaw, C.; Schoelermann, B.; Schulz, L.; Rabiller, M.; Bayer, P.; Hartmann-Fatu, C.
Human TPST1 transmembrane domain triggers enzyme dimerisation and localisation to the Golgi compartment (2006), J. Mol. Biol., 361, 436-449.

Search for more literature for 2.8.2.20

Cloned(Commentary)

Cloned (Comment)	Organism
expression of TPSt-1 in HeLa cells and COS-7 cells as GFP-tagged protein in the Golgi compartment	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	consruction of truncation mutants lacking the cytosolic or the transmembrane domain, the first are not affected in their subcellular localization in the Golgi compartment, the latter are mislocated in the cytosol, overview	Homo sapiens
N262A	site-directed mutagenesis, the N-glycosylation site mutant is less N-glycosylated and has a lower molecular weight compared to the wild-type enzyme	Homo sapiens
N60A	site-directed mutagenesis, the N-glycosylation site mutant is less N-glycosylated and has a lower molecular weight compared to the wild-type enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	the transmembrane domain of TPST1 is determined to act in targeting and retention of the enzyme to the trans-Golgi compartment	Homo sapiens	5794	-
membrane	transmembrane protein	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3'-phosphoadenylyl sulfate + protein tyrosine	Homo sapiens	-	adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	TPST1 bears N-linked glycosyl residues exclusively at position Asn60 and Asn262	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + protein tyrosine	-	Homo sapiens	adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate	-	?

Synonyms

Synonyms	Comment	Organism
TPST1	-	Homo sapiens
tyrosylprotein sulfotransferase	-	Homo sapiens

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Release 2023.1 (January 2023)