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Literature summary for 2.8.2.20 extracted from

  • Niehrs, C.; Huttner, W.B.
    Purification and characterization of tyrosylprotein sulfotransferase (1990), EMBO J., 9, 35-42.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic properties with synthetic peptides as substrates Bos taurus
0.0014
-
 3'-phosphoadenylylsulfate
-
 Bos taurus
0.021
-
 KKSAEDYEYPSMG pH 6.5, 30°C Bos taurus
0.37
-
 KKSAEDYQYPSMG pH 6.5, 30°C Bos taurus
0.55
-
 KKSAENYEYPSMG pH 6.5, 30°C Bos taurus
0.59
-
 KKSAENYQYPSMG pH 6.5, 30°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane trans-most subcompartment Bos taurus 139
-
membrane integral membrane-protein Bos taurus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 enzyme-detergent micelle, glycerol gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + protein tyrosine Bos taurus post-translational modification of biologically active peptides and proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bos taurus

Purification (Commentary)

Purification (Comment) Organism
treatment of membrane-bound enzyme with carbonate, followed by solubilization and affinity chromatography on a substrate peptide Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0057
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, detergent-solubilized enzyme preparation, t1/2: 48 h Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine specifically sulfates Tyr-residues adjacent to acidic amino acids Bos taurus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
3'-phosphoadenylylsulfate + alpha-tubulin peptide PKV Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + KKSAEDYQYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + KKSAENYEYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + KKSAENYQYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + PKG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + protein tyrosine post-translational modification of biologically active peptides and proteins Bos taurus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000-54000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 PKG Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5.7 6.8 about half-maximal activity at pH 5.7 and 6.8 Bos taurus